论文部分内容阅读
３０只雄性Ｗｉｓｔａｒ大鼠随机分为正常对照组（对照组）、糖尿病维生素Ｂ１２治疗组（治疗组）及糖尿病对照组（糖尿病组）。糖尿病大鼠模型用链脲佐菌素（ＳＴＺ）腹腔注射诱导。治疗组成模后８ｄ始肌肉注射甲基维生素Ｂ１２（５００μｇ／ｋｇ）其余两组注射等量生理盐水。实验６０ｄ结束时测定血糖及体重，断头处死大鼠采血测定睾酮及睾丸组织ｈＣＧ结合位点。结果显示对照组体重明显高于其余两组；而对照组血糖低于其余两组（Ｐ＜０．０１），治疗组与糖尿病组间体重及血糖无明显差异（Ｐ＞０．０５）。对照组睾酮高于其余两组（Ｐ＜０．０１），而治疗组又高于糖尿病组（Ｐ＜０．０５）；对照组ｈＣＧ结合位点显著高于其余两组（Ｐ＜０．０１），治疗组又高于糖尿病组（Ｐ＜０．０１）。结果提示糖尿病使大鼠睾酮分泌及睾丸组织ｈＣＧ结合位点均降低，而甲基维生素Ｂ１２对睾丸间质细胞功能及结构的维持有一定作用。维生素Ｂ１２对大鼠血糖及体重无明显影响。
Thirty male Wistar rats were randomly divided into normal control group (control group), diabetic B12 treatment group (treated group) and diabetic control group (diabetic group). Diabetic rat model was induced by intraperitoneal injection of streptozotocin (STZ). The rats in the treatment group were injected with methyl vitamin B12 (500μg / kg) intramuscularly on the 8th day after injection and the other two groups were given the same amount of saline. Blood glucose and body weight were measured at the end of the experiment 60d, blood samples were collected from decapitated rats to determine the hCG binding sites of testosterone and testicular tissue. The results showed that the body weight of the control group was significantly higher than the other two groups; while the control group had lower blood glucose levels than the other two groups (P <0.01). There was no significant difference in body weight and blood glucose between the treatment group and the diabetic group (P> 0.05). The testosterone level in the control group was higher than that in the other two groups (P <0.01), but was higher in the treatment group than in the diabetic group (P <0.05). The hCG binding site in the control group was significantly higher than the other two groups ), The treatment group was higher than the diabetic group (P <0.01). The results suggest that diabetes may decrease testosterone secretion and hCG binding sites in testis, while methylvitamin B12 may play a role in maintaining the function and structure of testicular stromal cells. Vitamin B12 had no significant effect on blood glucose and body weight in rats.