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目的：观察局部联合应用反义ｃｍｙｂ 、反义ｃＨａｒａｓ 对氮气干燥内皮剥脱术后大鼠颈动脉肌内膜增殖的抑制作用。方法：５０ 只ＳＤ 大鼠随机分为对照组、错配组、反义ｃｍｙｂ 组、反义ｃＨａｒａｓ 组和联合用药组，于内皮剥脱后经凝胶球囊局部导入不同核酸片段。术后１４ 天取材制片，采用计算机图像分析法测量管腔面积，内／ 中膜面积、厚度比值。结果：联合用药组管腔面积（０－３９ ±０－１２ ｍ ｍ２） 大于反义ｃｍｙｂ 、反义ｃＨａｒａｓ 组（０－２９ ±０－０１ ｍ ｍ ２ ，０－２８ ±０－０１ ｍ ｍ２ ，Ｐ＜ ０－０５） 和错配组（０－１８ ±０－０５ ｍ ｍ ２ ，Ｐ＜ ０－０１） ，与对照组（０－４０ ±０－１４ ｍ ｍ２） 比较，管腔面积无显著差异（ Ｐ＞ ０－０５） 。其内／ 中膜面积、厚度比值（０－１１ ±０－０２ ，０－０２ ±０－００） 均低于单一制剂组和错配组（０－４５ ±０－２４ ，０－３９ ±０－２５ ；０－５７ ±０－２４ ，０－５６ ±０－２３ ；１－１６ ±０－３８ ，１－８５ ±０－７９ ；Ｐ＜０－０１） 。结论：局部靶向联合应用反义ｃｍｙｂ 、反义ｃＨａｒａｓ 能进一步抑制血管损伤后肌内膜增殖，有效防止管腔狭窄
Objective: To observe the local combination of antisense c myb, antisense c Haras impact on carotid artery endarterectomy in rats after endarterectomy proliferation inhibition. Methods: Fifty SD rats were randomly divided into control group, mismatch group, antisense cmyb group, antisense cHaras group and combination group. After exfoliation of endothelial cells, different nucleic acids Fragment. 14 days after the preparation of the preparation, the use of computer image analysis of the lumen area, the inner / media area, thickness ratio. Results: The lumen area (0-39 ± 0-12 m m2) in the combination group was greater than that in the antisense cmyb and the antisense cHaras group (0-29 ± 0-01 m m 2, 0-28 ± 0-01 m m2, P <0-05) and the mismatch group (0-18 ± 0-05 m m2, P <0-01) compared with the control group (0-40 ± 0-14 m m2) , Lumen area no significant difference (P> 0-05). The ratio of intima / media area and thickness (0-11 ± 0-02, 0-02 ± 0-00) was lower than that of single preparation group and mismatch group (0-45 ± 0-24, 0-39 ± 0 -25; 0-57 ± 0-24, 0-56 ± 0-23; 1-16 ± 0-38, 1-85 ± 0-79; P <0-01). Conclusion: The combination of local targeting antisense c myb, antisense c Haras can further inhibit endometrial proliferation after vascular injury, effectively prevent stenosis