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目的分析新疆汉族、维吾尔族和其他民族2型糖尿病患者尿液中氨基酸谱的差异。方法用高效液相色谱法,以甲醇-乙腈-水和醋酸钠溶液(p H6.5)为流动相梯度洗脱;用荧光检测器对尿液中游离氨基酸进行含量测定。结果汉族2型糖尿病组尿液中的天冬氨酸、谷氨酸、缬氨酸、甘氨酸、亮氨酸、异亮氨酸、苯丙氨酸含量显著高于维吾尔族糖尿病组(P<0.05),亮氨酸和异亮氨酸的含量显著高于其他民族2型糖尿病患者(P<0.05),而脯氨酸和胱氨酸的含量显著低于其他民族2型糖尿病组(P<0.05)。结论新疆汉族与维吾尔族、其他民族2型糖尿病患者尿液中氨基酸水平存在显著差异。
Objective To analyze the differences of amino acid profiles in urine of type 2 diabetic patients in Han, Uighur and other ethnic groups in Xinjiang. Methods High performance liquid chromatography (HPLC) with gradient elution of methanol-acetonitrile-water and sodium acetate solution (p H6.5) was used as the mobile phase. The content of free amino acids in urine was determined by fluorescence detector. Results The urinary contents of aspartate, glutamate, valine, glycine, leucine, isoleucine and phenylalanine in Han type 2 diabetic patients were significantly higher than those in Uygur diabetic patients (P <0.05 ), Leucine and isoleucine were significantly higher than those in other ethnic type 2 diabetes mellitus (P <0.05), while proline and cystine contents were significantly lower than those in other ethnic type 2 diabetic patients (P <0.05 ). Conclusion There are significant differences in urinary amino acid levels between Han and Uygur nationality in Xinjiang and other ethnic groups with type 2 diabetes mellitus.