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前言通常应用的液相色谱柱的柱径为4mm,柱长为15～30cm;近年来的趋势是采用更短的色谱柱(柱长为5～10cm)以及更细的柱径(φ0.5～1mm)。这种短而细的色谱柱的优点很明显:短柱的柱压远低于长柱,降低了对高压的要求,相应地降低了仪器的造价;此外,采用短柱,也使分离时间缩短,有利于快速分析。细径柱的优点是流动相耗费量少,0.5mm的柱子所消耗的流动相仅为常规4mm柱的1/100左右。但更短的微型柱的应用却很少报导。
Foreword Commonly used liquid chromatography columns have a column diameter of 4 mm and a column length of 15 to 30 cm. In recent years, the trend has been to use shorter columns (column lengths of 5 to 10 cm) and finer column diameters ~ 1mm). The advantages of this short, narrow column are obvious: the column pressure is much lower than the column, reducing the need for high pressure, which in turn reduces the cost of the instrument; in addition, the use of short columns also shortens the separation time , Is conducive to rapid analysis. The advantage of small diameter columns is that the mobile phase consumes less, and the mobile phase consumed by a 0.5 mm column is only about 1/100 of a conventional 4 mm column. However, the application of shorter microcolumns is seldom reported.