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目的探讨B淋巴细胞瘤-2(B-cell lymphoma-2,Bcl-2)蛋白和髓细胞白血病因子-1(myeloid cell leukemia-1,Mcl-1)蛋白在神经母细胞瘤组织中的表达及意义。方法神经母细胞瘤患者40例为神经母细胞瘤组,神经节细胞瘤患者10例为对照组,均行手术治疗,采用免疫组织化学法检测2组手术切除瘤组织中Bcl-2、Mcl-1蛋白阳性表达情况,分析Bcl-2、Mcl-1蛋白阳性表达与神经母细胞瘤临床病理特征的关系。结果神经母细胞瘤组组织中Bcl-2、Mcl-1蛋白阳性表达率(67.50%、60.00%)均高于对照组(10.00%、10.00%)(P<0.05);神经母细胞瘤组Bcl-2、Mcl-1蛋白阳性表达率在年龄≥1岁(75.00%、66.67%)、有淋巴结转移(78.94%、68.42%)、中低分化者(72.72%、63.64%)高于年龄<1岁(56.25%、50.00%)、无淋巴结转移(57.14%、52.38%)、高分化者(42.85%、42.85%)(P<0.05);临床分期Ⅰ~Ⅱ期患者Bcl-2、Mcl-1蛋白阳性表达率(66.67%、61.11%)与Ⅲ~Ⅳ期患者(68.18%、59.09%)比较差异无统计学意义(P>0.05)。结论神经母细胞瘤组织中Bcl-2、Mcl-1蛋白呈高表达,可能与神经母细胞瘤的侵袭、淋巴结转移有关。
Objective To investigate the expression of Bcl-2 protein and myeloid cell leukemia-1 (Mcl-1) protein in neuroblastoma tissue, significance. Methods 40 patients with neuroblastoma were neuroblastoma group and 10 patients with ganglioneuroma as control group. All patients underwent surgical treatment. Immunohistochemistry was used to detect the expression of Bcl-2 and Mcl- 1 protein positive expression, analysis of Bcl-2, Mcl-1 protein expression and neuroblastoma clinicopathological features. Results The positive rates of Bcl-2 and Mcl-1 in neuroblastoma tissues were significantly higher than those in control group (10.00%, 10.00%, 10.00%, respectively) (P <0.05) The positive rates of Mcl-1 and Mcl-1 were higher than those with age <1 (75.00%, 66.67%), lymph node metastasis (78.94%, 68.42%) and moderately poorly differentiated (72.72%, 63.64% (56.25%, 50.00%), no lymph node metastasis (57.14%, 52.38%), well differentiated (42.85%, 42.85%) (P <0.05). The levels of Bcl-2 and Mcl-1 The positive expression rate of protein (66.67%, 61.11%) was not significantly different from that of stage Ⅲ ~ Ⅳ (68.18%, 59.09%) (P> 0.05). Conclusion The high expression of Bcl-2 and Mcl-1 in neuroblastoma may be related to the invasion and lymph node metastasis of neuroblastoma.