【摘 要】
：
目的 探索实体瘤序贯双次移植患者自体外周血造血干细胞采集方法及安全性,分析采集效果.方法 选择2019年1月～2021年6月行细胞采集的27名实体瘤患者为研究对象,重组人粒细胞刺激因子及重组人粒细胞巨噬细胞集落刺激因子动员后行采集术.制订个性化方案,采集前全面评估患儿病情及配合程度,建立深静脉通路,以保证采集顺利进行.选择单核细胞采集(MNC)程序,设置机器参数.采集结束后取混匀的标本进行单个核细胞计数及CD34+细胞计数,做回顾性分析.结果 27名患者共进行细胞采集73次,获得的单个核细胞数为12.58
【机 构】
：
北京京都儿童医院血液科,北京102208
论文部分内容阅读
目的 探索实体瘤序贯双次移植患者自体外周血造血干细胞采集方法及安全性,分析采集效果.方法 选择2019年1月～2021年6月行细胞采集的27名实体瘤患者为研究对象,重组人粒细胞刺激因子及重组人粒细胞巨噬细胞集落刺激因子动员后行采集术.制订个性化方案,采集前全面评估患儿病情及配合程度,建立深静脉通路,以保证采集顺利进行.选择单核细胞采集(MNC)程序,设置机器参数.采集结束后取混匀的标本进行单个核细胞计数及CD34+细胞计数,做回顾性分析.结果 27名患者共进行细胞采集73次,获得的单个核细胞数为12.586(10.22～19.586)×108/kg,CD34+细胞数为13.575(7.275～23.825)×106/kg,满足双次自体造血干细胞移植所需.未发生枸橼酸中毒及其他严重不良反应,随访一般状况良好.结论 我们的采集方法对儿童是有效和安全的,即使体重小也可推荐使用,能为实体瘤患儿采集足够的干细胞,满足序贯双次自体造血干细胞移植需要.
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