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目的：研究血管内皮生长因子 C(VEGF-C)在大肠癌组织中的表达水平及其与大肠癌临床病理特征、微 血管密度(MVD)及预后的关系。方法：抽取1996年1月～1998年1月行结直肠癌根治术且接受正规随访的大肠癌 病例69例；采用 EnVision 免疫组织化学技术检测 VEGF-C 在69例大肠癌及20例正常大肠组织的表达；采用抗 CD34免疫组织化学技术评价大肠癌 MVD；采用 Axioplan 2 Imaging 显微图像分析系统对免疫组织化学染色结果进 行定量。结果：94． 2%(65/69) 大肠癌组织和30%(6/20) 正常大肠组织检测到 VEGF-C 表达，染色定位于肿瘤细胞浆； 大肠癌 VEGF-C 表达显著高于相应正常大肠组织(P＜0． 01) ，且与大肠癌淋巴结转移、MVD 及临床分期显著相关(P＜ 0． 05) ，而与病人年龄(≤68；＞68岁)、性别、肿瘤浸润深度、远处转移、分化程度及肿瘤部位相关性均无统汁学意义； VEGF-C 高表达的病人组总生存率和无病生存率均显著低于 VEGF-C 低表达病人组(P＜0． 05) 。结论：VEGF-C 在大肠 癌中异常高表达，且与大肠癌淋巴结转移、临床分期、无病生存率与总生存率显著相关，可作为大肠癌预后判断标记物。
Objective: To investigate the expression of vascular endothelial growth factor C (VEGF-C) in colorectal carcinoma and its relationship with clinicopathological characteristics, microvessel density (MVD) and prognosis of colorectal cancer. Methods: Totally 69 cases of colorectal cancer undergoing radical resection of colorectal cancer from January 1996 to January 1998 were enrolled in this study. EnVision immunohistochemistry was used to detect the expression of VEGF-C in 69 cases of colorectal carcinoma and 20 cases of normal colorectal tissue The expression of MVD in colorectal cancer was evaluated by anti-CD34 immunohistochemistry. The Axioplan 2 Imaging microscopic image analysis system was used to quantify the immunohistochemical staining results. Results: 94. The expression of VEGF-C was detected in 2% (65/69) and 30% (6/20) of normal colorectal tissues, and the staining was located in tumor cytoplasm. The expression of VEGF-C in colorectal cancer was significantly higher than that in normal colorectal tissue (P <0.05), but not with age (≤68;> 68 years), gender, depth of tumor invasion, distant metastasis, lymph node metastasis, lymph node metastasis, There was no significant difference in the degree of differentiation and the correlation between the tumor sites. The overall survival and disease-free survival of patients with VEGF-C overexpression were significantly lower than those with low VEGF-C expression (P <0.05). CONCLUSION: VEGF-C is highly expressed in colorectal cancer and is significantly associated with lymph node metastasis, clinical stage, disease-free survival and overall survival in colorectal cancer and may be used as a marker of prognosis in colorectal cancer.