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本研究采用 ＰＣＲ法从中国妇女鲍温氏病（Ｂｏｗｅｎ＇ｓ ｄｉｓｅａｓｅ）组织标本扩增获得了与宫颈癌密切相关的人乳头 瘤病毒１６型（ＨＰＶ１６）的晚期基因Ｌ１，并装入测序载体测序分析了其ＤＮＡ的序列，与标准型进行了比较，发现有 若干变异。此研究对序列及变异意义进行了分析和讨论，为今后Ｌ１基因分子流行病学调查、Ｌ１蛋白的结构与功能研 究、疫苗研制以及ＨＰＶ相关的基础性研究提供了有用的资料。同时在国内外首次报道了鲍温氏病组织中ＨＰＶ１６Ｌ１的 序列及其变异情况，并在ＧｅｎＢａｎｋ［１］申请到序列号（ＡＦ０８９５２）。
In this study, a late-stage gene L1 of human papillomavirus type 16 (HPV16) closely related to cervical cancer was amplified by PCR from Chinese Bowen’s disease tissue samples and sequenced. The sequence of its DNA was analyzed and compared with the standard one and found that there were some variations. This study analyzed and discussed the significance of sequence and mutation, and provided useful information for the molecular epidemiological investigation of L1 gene, the structure and function of L1 protein, the development of vaccine and the basic research on HPV. At the same time, the sequence and variation of HPV16L1 in Bowen’s disease were reported for the first time both in China and abroad. The sequence number (AF08952) was applied in GenBank [1].