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缺血性脑血管疾病的干细胞治疗已成热点。VEGF能诱导血管生成、增加血管通透性及维持血管功能,广泛应用缺血性疾病的治疗研究。然而VEGF难以在移植局部维持足够的浓度,寻找一种能够在移植局部持续高表达VEGF的方法是促进VEGF临床应用的关键。将VEGF编码基因转染干细胞进行基因修饰是获得血液循环重建最具发展潜力的治疗模式。本文从VEGF的生物特性及调节因素出发,比较干细胞载体的特点及不同的转染方法,展望其在缺血性脑血管疾病临床应用的前景。
Stem cell therapy for ischemic cerebrovascular disease has become a hot spot. VEGF can induce angiogenesis, increase vascular permeability and maintain vascular function, widely used in the treatment of ischemic disease research. However, it is difficult for VEGF to maintain a sufficient concentration in the local area of transplantation. Therefore, finding a method capable of continuously expressing VEGF in the transplant site is the key to promote the clinical application of VEGF. Transfection of VEGF-encoding genes into stem cells for gene modification is the most promising therapeutic modality for achieving blood circulation reconstruction. In this paper, based on the biological characteristics and regulatory factors of VEGF, we compared the characteristics of stem cell vectors and different transfection methods and prospected their clinical application in ischemic cerebrovascular diseases.