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目的 :探讨了重原子盐Cd(Ac) 2 和Zn(Ac) 2 对 6 -巯基嘌呤室温磷光 (RTP)的影响。方法 :配制一定浓度的 6 -巯基嘌呤溶液 ,选择不同的固体基质及酸度条件 ,测 2种重原子Cd2 + 、Zn2 + 盐存在下RTP。结果 :适宜固体基质为滤纸 ,pH范围 1～ 8有较强的RTP信号 ,Cd(Ac) 2 、Zn(Ac) 2 对 6 -巯基嘌呤RTP有较强增敏作用。以Cd(Ac) 2 溶液作重原子微扰剂时 ,6 -巯基嘌呤的线性范围为 6× 10 -7～ 1× 10 -3 mol·L-1,检出限为每斑点 6 5ng ;以Zn(Ac) 2 溶液作重原子微扰剂时 ,6 -巯基嘌呤的线性范围为 6× 10 -7～ 1× 10 -3 mol·L-1,检出限为每斑点 4 8ng。 结论 :Cd(Ac) 2 、Zn(Ac) 2 可诱导 6 -巯基嘌呤产生强的RTP ,且Cd(Ac) 2 检出限优于Zn(Ac) 2 。本方法简便 ,快速 ,选择性好 ,线性范围宽 ,检出限低 ,结果准确可靠
Objective: To investigate the effects of heavy atom salts Cd (Ac) 2 and Zn (Ac) 2 on room temperature phosphorescence (RTP) of 6 - mercaptopurine. Methods: A certain concentration of 6 - mercaptopurine solution was prepared. Different solid substrates and acidity conditions were selected to determine RTP in the presence of two heavy atoms Cd2 + and Zn2 +. Results: The suitable solid substrate was filter paper, which had a strong RTP signal in the range of 1 ~ 8. The Cd (Ac) 2 and Zn (Ac) 2 sensitized RTP to 6 - mercaptopurine. The linear range of 6 - mercaptopurine was 6 × 10 -7 ~ 1 × 10 -3 mol·L -1 with Cd (Ac) 2 as heavy atom perturbation agent, the detection limit was 65 ng per spot. The linear range of 6 - mercaptopurine was 6 × 10 -7 ~ 1 × 10 -3 mol·L -1 with Zn (Ac) 2 as heavy atom perturbation agent, and the detection limit was 48 ng per spot. Conclusion: Cd (Ac) 2 and Zn (Ac) 2 can induce strong RTP of 6 - mercaptopurine, and the detection limit of Cd (Ac) 2 is better than that of Zn (Ac) 2. The method is simple, rapid, good selectivity, wide linear range, low detection limit, the results are accurate and reliable