论文部分内容阅读
目的建立高效液相色谱法同时测定人血浆中全反式维甲酸、13-顺式维甲酸和9-顺式维甲酸浓度。方法色谱柱为KromasilC18柱(4.6mm×250mm,5μm);柱温为室温。流动相A:甲醇;流动相B:0.01mol/L醋酸钠缓冲液(pH=5.7),梯度洗脱,流速1ml/min;检测波长340nm。结果全反式维甲酸、13-顺式维甲酸和9-顺式维甲酸血药浓度在1～200ng/ml范围内,浓度与峰面积比有良好的线性关系,最低检测浓度为0.5ng/ml。全反式维甲酸方法回收率为97.22%～108.80%,日内RSD≤8.24%,日间RSD≤11.34%;13-顺式维甲酸方法回收率为98.62%～104.80%,日内RSD≤8.02%,日间RSD≤11.70%;9-顺式维甲酸方法回收率为97.74%～102.24%,日内RSD≤7.72%,日间RSD≤9.17%。结论本方法简单、快速、灵敏、重现性好,适用于全反式维甲酸、13-顺式维甲酸和9-顺式维甲酸临床血药浓度监测及人体药代动力学研究。
Objective To establish a HPLC method for simultaneous determination of all-trans retinoic acid, 13-cis retinoic acid and 9-cis retinoic acid in human plasma. Methods The column was Kromasil C18 column (4.6 mm × 250 mm, 5 μm). The column temperature was room temperature. Mobile phase A: methanol; mobile phase B: 0.01mol / L sodium acetate buffer (pH = 5.7), gradient elution, flow rate 1ml / min; detection wavelength 340nm. Results The concentrations of all-trans retinoic acid, 13-cis retinoic acid and 9-cis retinoic acid ranged from 1 ng / ml to 200 ng / ml with a good linear relationship with the peak area ratio. The lowest detection concentration was 0.5 ng / ml. The recovery rate of all-trans retinoic acid method was 97.22% -108.80%, RSD≤8.24%, daytime RSD≤11.34%. The recoveries of 13-cis retinoic acid method were 98.62% ~ 104.80%, RSD≤8.02% Day RSD≤11.70%; 9-cis retinoic acid recovery rate was 97.74% ~ 102.24%, RSD≤7.72%, daytime RSD≤9.17%. Conclusion The method is simple, rapid, sensitive and reproducible. It is suitable for the monitoring of clinical blood concentration of all-trans retinoic acid, 13-cis-retinoic acid and 9-cis retinoic acid and the pharmacokinetics of human.