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以往组织培养时从产生愈伤组织、分化成苗到生根都是在培养基上完成.其中,组培幼苗生根比较困难。本试验采用将百脉根无根苗移栽于土壤刺激生根的方法,成活率达70％以上,移栽后7周左右可全部生根,其繁殖系数增加43.7倍,为珍贵材料的快速繁殖开辟了一条新途径。
In the past from the generation of tissue culture callus, differentiation into seedlings to rooting are completed in the medium, of which the tissue culture seedling rooting is more difficult. In this experiment, the root-free seedlings of Lotus japonicus were transplanted in the soil to stimulate the rooting. The survival rate was above 70%. After 7 weeks of transplanting, all the roots could be rooted and its multiplication coefficient increased 43.7 times, which opened up the rapid propagation of precious materials A new way.