胆管闭锁婴儿肝脏组织中免疫球蛋白沉积与巨细胞病毒感染间关系

• 作 者: Fischler B. Woxenius S. Nemeth A. Papadogiannakis N. 宁亮
• 机 构: Department of Pediatrics Huddinge University Hospital Karolinska Institutet SE- 141 86 Stockholm Sw
• 出 处: 世界核心医学期刊文摘(儿科学分册)
• 发表日期: 2005年08期
• 关键词: 胆管闭锁 巨细胞病毒感染 免疫球蛋白 婴儿 肝脏 活检组织 沉积情况 免疫组化染色 肝脏疾病 肝细胞

Purpose: The aim of this report was to study the amount and distribution of i mmunoglobulin deposits in liver biopsies from infants with biliary atresia (BA) and correlate the results to the cytomegalovirus (CMV) infection status. Methods : Stored liver biopsies from 18 patients with BA and from 6 control patients wit hout liver disease were immunohistochemically stained to detect IgG and IgM depo sits. The intensity of the immunoglobulin staining was evaluated by a semiquanti tative scoring scale. Ongoing CMV infection was defined as the detection of CMV - IgM in serum and/or the isolation of CMV in the urine and was noted in 9 of t he patients with BA. Results: When analyzing the immunoglobulin deposits on the hepatocellular canalicular membrane the intensity score for IgM deposits was sig nificantly higher in biopsies from patients with BA infected with CMV than in th ose without. No canalicular staining was detected in control biopsies. Conclusio ns: The results support the possibility that immunologic mechanisms are of impor tance in the pathogenesis of BA and that a CMV infection may trigger such mechan isms.

Purpose: The aim of this report was to study the amount and distribution of i mmunoglobulin deposits in liver biopsies from infants with biliary atresia (BA) and correlate the results to the cytomegalovirus (CMV) infection status. Methods: Stored liver biopsies from 18 patients with BA and from 6 control patients wit hout liver disease were immunohistochemically stained to detect IgG and IgM depo sits. The intensity of the immunoglobulin staining was evaluated by a semiquanti tative scoring scale. Ongoing CMV infection was defined as the detection of CMV-IgM in serum and / or the isolation of CMV in the urine and was noted in 9 of t he patients with BA. Results: When analyzing the immunoglobulin deposits on the hepatocellular canalicular membrane the intensity score for IgM deposits was sig nificantly higher in biopsies from patients with BA infected with CMV than in th ose without. No canalicular staining was detected in control biopsies. Conclusio ns: The results support the possibi lity that immunologic mechanisms are of impor tance in the pathogenesis of BA and that a CMV infection may trigger such mechanisms.