论文部分内容阅读
枯草芽孢杆菌（Ｂａｃｉｌｕｓｓｕｂｔｉｌｉｓ）在不同培养基、不同培养时间、不同温度、不同ｐＨ值、不同碳源和氮源条件下，产生抗菌物质的量有显著差异。在ＮＢ和Ｍ９两种培养液中，Ｂｐ对水稻白叶枯病菌及小麦赤霉病菌的抑菌效价随时间延长而提高，培养１２０ｈ后，仍继续上升。在３０℃，以ｐＨ９的ＮＢ培养的Ｂｐ对水稻白叶枯病菌的抑菌效价较高；而对小麦赤霉病菌的抑菌效价，又以２０℃，ｐＨ６～９的ＮＢ培养时较高。在所测定的９种不同碳源、６种不同氮源中，以终浓度为０．２％的可溶性淀粉、０．２％的甘露醇或０．１％的葡萄糖作碳源，０．１％的硫酸铵、氯化铵、硝酸铵或半胱氨酸为氮源有助于Ｂｐ抗菌物质的产生。
Bacillus subtilis (Bacilussubtilis) in different media, different culture time, different temperatures, different pH values, different carbon sources and nitrogen sources, the amount of antibacterial substances were significantly different. In NB and M9 medium, the antibacterial activity of Bp against the bacterial leaf blight and Gibberella zeae was increased with time, and continued to increase after 120 hours. At 30 ℃, the antibacterial activity of Bp cultured on NB9 at pH9 was higher than that of Xanthomonas oryzae at 30 ℃. However, the antibacterial activity of Bp against Gibberella zeae at 20 ℃ and NB6 of 9 high. Among the 9 different carbon sources and 6 different nitrogen sources tested, 0.2% of soluble starch, 0.2% of mannitol or 0.1% of glucose was used as carbon source and 0.1 % Ammonium sulfate, ammonium chloride, ammonium nitrate or cysteine as a nitrogen source contributes to the production of Bp antibacterial substances.