结球白菜组培苗不经低温条件在试管内抽薹开花

• 作 者: 张伟丽 钮心恪 王增贵
• 机 构: 中国农科院蔬菜花卉所,中国农科院蔬菜花卉所,山东省农科院原子能应用研究所 北京100081,在读研究生,北京100081
• 出 处: 中国蔬菜
• 发表日期: 1993年05期
• 关键词: 结球白菜 抽薹开花 培苗 自交不亲和系 丛生芽 春化阶段 继代 生长发育特性 芽组织 下胚轴

用结球白菜自交不亲和系91-352作试材。该系为山东省农科院蔬菜所用72与B77-206杂交获得的早代系,冬性中等。其种子接于无菌培养基7天后,取发芽小苗切除下胚轴,用其余部分为外植体进行诱芽组织培养。在温度25±2℃、光照2000lX、每日光照时间 12小时条件下,经 25～ 30天培养后,

Chinese cabbage selfing with incompatible lines 91-352 for the test material. The Department of Shandong Academy of Agricultural Sciences vegetables used 72 and B77-206 obtained early hybrids, winter moderate. The seeds were taken in sterile culture medium for 7 days, the germinated seedlings were taken to excise the hypocotyls and the rest were used as explants to induce bud tissue culture. At a temperature of 25 ± 2 ℃, light 2000lX, daily light conditions for 12 hours under the conditions of 25 to 30 days after culture,