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癌基因分离的一个重要手段是通过用高分子量DNA转染NIH3T3细胞。通过挑选具有形态改变和重叠生长的转化灶来识别恶性转化细胞。ras基因家族能诱导NIH3T3细胞形成转化灶,而用DNA转染技术从人的肿瘤或肿瘤细胞系中分离到的转化基因只有ras家族等几种癌基因,象mye及其它一些癌基因不能使NIH3T3细胞发生明显的形态改变,因此用这一筛
An important means of oncogene isolation is through the transfection of NIH3T3 cells with high molecular weight DNA. Malignant transformed cells are identified by selecting foci that have morphological changes and overlapping growth. The ras gene family can induce the NIH3T3 cells to form a foci while the transformants isolated from human tumors or tumor cell lines by DNA transfection are only a few of the ras family oncogenes. Mye and some other oncogenes can not make NIH3T3 The cells undergo significant morphological changes, so use this sieve