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目的研究不同人工诱导方法对白木香结香过程中萜类相关基因表达量的影响。方法 “小孔滴注法”进行甲酸、甲酸结合镰刀菌人工诱导试验,手工割取诱导前、后12个月内的白木香结香部位,实时荧光定量PCR法(qRT-PCR)测定As-HMGR、As-DXS1、As-DXS2基因表达水平。结果 As-HMGR、As-DXS1、As-DXS2回归方程分别为Y=-3.580X+40.11、Y=-3.424X+38.38、Y=-3.728X+41.84,相关系数均大于0.997,线性关系均良好。甲酸、甲酸结合镰刀菌诱导过程中,As-DXS1与As-DXS2的表达模式相近,前8个月表达水平较低,随后显著升高。甲酸诱导时,As-HMGR基因从2个月开始表达,呈现先升高后下降再升高的趋势;甲酸结合镰刀菌诱导时,初期相对表达量缓慢升高,6个月时达到峰值,随后急剧下降。人工诱导12个月As-HMGR、As-DXS1的相对表达量,甲酸诱导高于甲酸结合镰刀菌诱导(P<0.05)。结论甲酸、甲酸结合镰刀菌诱导白木香结香过程中As-HMGR基因在伤害早期响应伤害胁迫,As-DXS1、As-DXS2基因在后期响应,为进一步研究白木香萜类次生代谢产物表达与调控,改善人工沉香品质奠定基础。
Aim To study the effects of different artificial induction methods on the expression of terpenoid related genes in the process of pilchard incense. Methods Formic acid and formic acid combined with Fusarium artificial induction test were conducted by hand drop instillation method. The extracts were collected manually by qRT-PCR (qRT-PCR) As-HMGR, As-DXS1, As-DXS2 gene expression levels. Results The regression equations of As-HMGR, As-DXS1 and As-DXS2 were Y = -3.580X + 40.11, Y = -3.424X + 38.38, Y = -3.728X + 41.84, respectively, and the correlation coefficients were all greater than 0.997 with good linearity . During the induction of formic acid and formic acid into Fusarium, the expression patterns of As-DXS1 and As-DXS2 were similar and the expression levels of As-DXS1 and As-DXS2 were lower in the first 8 months and then significantly increased. When induced by formic acid, As-HMGR gene began to express at 2 months, showing a tendency of first increasing, then decreasing and then increasing. When induced by fusaric acid, initial relative expression slowly increased and peaked at 6 months, A sharp decline. The relative expression of As-HMGR and As-DXS1 induced by artificial induction for 12 months was higher than that induced by formic acid induced by Fusobacterium formate (P <0.05). CONCLUSIONS Formic acid and formic acid combined with Fusarium spp. Induce As-HMGR gene response to injury injury in the early stage of injury, and As-DXS1 and As-DXS2 genes respond in the late stage. In order to further study the expression of the secondary metabolites of strychni, Regulation, improve the quality of artificial incense lays the foundation.