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Saito等(1980)介绍一种新方法,来检验传统鸡蛋培养基上生长的各种分枝杆菌的热稳定酸性磷酸酶活性,以鉴别这些分枝杆菌。在该试验中,沉淀的细菌转变成红色即为阳性,但这种结果有时不易判定。本文报道了一种快速、简便的改良热稳定酸性磷酸酶试验,其阳性结果是出现一种鲜明的深紫红色,且色泽不仅存在于沉淀的细菌中,也弥漫于整个悬液,因此很容易鉴别。另外为方便起见,本试验可与68℃触酶试验同时进行。试验方法如下:取L(?)wenstein-Jensen培养基上生长一个月的细菌2接种环,加入盛有0.3ml灭菌缓冲液(pH7)的试管(13×
Saito et al. (1980) introduced a new method to examine the thermostable acid phosphatase activity of various mycobacteria grown on traditional egg culture medium to identify these mycobacteria. In this test, the precipitation of bacteria into red is positive, but this result is sometimes difficult to determine. In this paper, we report a fast, simple, modified thermostable acid phosphatase assay with a positive result of a deep purplish red color that is not only found in precipitated bacteria but also throughout the suspension and is therefore very easy Identification. Also for convenience, this test can be carried out simultaneously with the 68 ℃ catalase test. The test method was as follows: a bacterial 2 inoculation loop grown for one month on L (?) Wenstein-Jensen medium was added to a test tube (13 ×, containing 0.3 ml of sterilizing buffer (pH 7)