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白芍总甙（ＴＧＰ）对脂多糖（ＬＰＳ）诱导的小鼠脾淋巴细胞增殖反应的量效曲线呈钟形，用贴壁法除去脾细胞中巨噬细胞（ＭΦ）或加入１０μｍｏｌ·Ｌ１吲哚美辛（Ｉｎｄ）可使ＴＧＰ量效曲线的下降支消失，再加５％同系小鼠腹腔ＭΦ中或前列腺素Ｅ２（ＰＧＥ２）０．０２２０μｍｏｌ·Ｌ１可使量效曲线下降支再现．同步检测ＴＧＰ对ＬＰＳ诱导大鼠腹腔ＭΦ产生ＰＧＥ２与白介素１（ＩＬ１）．结果表明，ＴＧＰ０．５－３ｌ２．５μｇ·ｍＬ１对ＬＰＳ诱导的ＩＬ－１产生曲线呈钟形．而ＴＧＰＬＰＳ的ＰＧＥ２产生曲线呈浓度依赖性地增高；在１２．５－３１２．５μｇ·ｍＬＴＧＰ范围内，１０μｍｏｌ·Ｌ１Ｉｎｄ可使高浓度ＴＣＰＬＰＳ的ＩＬ－１释放曲线明显抬高。提示ＴＧＰ对ＬＰＳ诱导的Ｂ细胞增殖反应和ＩＬ－１诱生的负调节作用都与其促进ＭΦ释放ＰＧＥ２有关．
The dose-response curves of total glucosides of paeony glycosides (TGP) on splenic lymphocyte proliferation induced by lipopolysaccharide (LPS) in mice were bell-shaped, macrophages (MΦ) in spleen cells were removed by adherent method or 10μmol·L1 indole Indometacin could decrease the descending branch of TGP dose-response curve, and the dose-response curve descended with the addition of 0.0220 μmol·L1 in the peritoneal MΦ of 5% homologous mice or PGE2. Simultaneous detection of TGP on LPS-induced peritoneal MΦ production of PGE2 and interleukin-1 (IL1). The results showed that, TGP0.5-3l2.5μg · mL1 LPS-induced IL-1 curve was bell-shaped. While TGPLPS PGE2 production curve increased in a concentration-dependent manner; in the range of 12.5-312.5μg · mLTGP, 10μmol·L1Ind could significantly increase IL-1 release curve of high concentration TCPLPS. It is suggested that TGP can induce LPS-induced B cell proliferation and negative regulation of IL-1 induction, which is related to the promotion of PGP2 release by MΦ.