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目的:探讨草分子杆菌(Mycobacterium Phlei)对SPCA/Ⅰ人肺腺癌细胞株的抗增殖效应及诱导细胞凋亡的作用。方法:将SPCA/Ⅰ人肺腺癌细胞株培养传代,分别加入不同浓度的草分子杆菌,作用36h。分别以酶标仪检测细胞抑制率,FACSAria流式细胞仪检测细胞凋亡率,倒置显微镜观察凋亡细胞形态。结果:不同浓度的草分子杆菌体外对人肺腺癌细胞株SPCA/Ⅰ均有直接的抑制作用,其抑制率与所用药物浓度呈正相关。草分子杆菌体外对人肺腺癌细胞株SPCA/Ⅰ有诱导凋亡的作用,细胞凋亡率明显增加。倒置显微镜下可见细胞由原来贴壁生长而逐渐脱落、外形变圆、体积缩小、折光性增强,核染色质凝集,细胞裂解。结论:草分子杆菌能通过诱导细胞凋亡,直接抑制人肺腺癌细胞株SPCA/Ⅰ增殖。
Objective: To investigate the antiproliferative effect of Mycobacterium Phlei on SPCA / Ⅰ human lung adenocarcinoma cell line and the effect of inducing apoptosis. Methods: The SPCA / Ⅰ human lung adenocarcinoma cell lines were cultured and passaged, respectively, and different concentrations of S. aureus were added for 36h. Cell inhibition rate was detected by microplate reader. FACSSAria flow cytometry was used to detect the apoptosis rate. The morphology of apoptotic cells was observed by inverted microscope. Results: Different concentrations of A. herbicum directly inhibited human lung adenocarcinoma cell line SPCA / Ⅰ in vitro, and the inhibition rate was positively correlated with the drug concentration. Gram-positive bacilli induced apoptosis in human lung adenocarcinoma cell line SPCA / Ⅰ in vitro, and the apoptosis rate was significantly increased. Under inverted microscope, the cells gradually shed from the original adherent growth, rounded shape, reduced size, enhanced refraction, nuclear chromatin condensation, cell lysis. CONCLUSION: S. grass can directly inhibit the proliferation of human lung adenocarcinoma cell line SPCA / Ⅰ by inducing apoptosis.