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目的: 探讨乳黄制剂对A型肝性脑病大鼠皮层GABAA受体表达的影响。方法:用硫代乙酰胺 0、24h灌胃建立大鼠A型肝性脑病模型, 乳黄制剂分大、中、小三个剂量组, 以乳果糖组为对照, 干化学法检测血氨水平, 高效液相色谱法检测血清、脑组织GABA含量, 免疫组织化学法观察皮层GABAA受体表达的变化。结果: 模型组血氨、血清与脑组织GABA含量、大脑皮层GABAA受体阳性细胞数和平均光密度均显著高于正常组, 乳黄制剂三个剂量组均较模型组有所下降, 其中, 乳黄制剂中剂量组下降最显著。结论: 肝性脑病的发生与GABA及GABAA受体增加密切相关, 乳黄制剂能明显降低A型肝性脑病大鼠GA BA含量, 减少GABA 受体的表达, 从而有效防治肝性脑病。
Objective: To investigate the effect of Huanghuang preparation on the expression of GABAA receptor in cortex of rats with type A hepatic encephalopathy. METHODS: A rat model of hepatic encephalopathy was established by intragastric administration of thioacetamide for 0 and 24 h. The formulation was divided into large, medium, and small dose groups. The lactulose group was used as a control, and the dry ammonia level was determined by dry chemical method. The content of GABA in serum and brain tissue was detected by HPLC, and the expression of GABAA receptor in cortex was detected by immunohistochemistry. RESULTS: Blood ammonia, serum and brain GABA content, number of GABAA receptor positive cells and average optical density in the cerebral cortex were significantly higher in the model group than in the normal group. The three doses of the milk fat formulation were lower than those in the model group. The most significant decrease was in the dose group in the cream formulae. Conclusion: The occurrence of hepatic encephalopathy is closely related to the increase of GABA and GABAA receptors. Huanghuang preparation can significantly reduce GABA content in rats with type A hepatic encephalopathy and reduce the expression of GABA receptors, thus effectively preventing hepatic encephalopathy.