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目的:探讨存活素基因在膀胱移行细胞癌中的表达,以及存活素基因表达与癌线胞的凋亡和增值活性的关系。方法:应用Western blot定量分析检测存活素蛋白在128例膀胱移行细胞瘤组织中的表达水平,原位DNA片段末端标记法检测肿瘤细胞的凋亡指数(Al),Ki-67单克隆抗体检测肿瘤细胞的增殖活性(Ki-67LI)。结果:Wstern blot分析结果表明在肿瘤组织中可检测到存活素的高表达,而正常膀胱组织中无存活素蛋白的表达。存活素蛋白的表达水平在肿瘤的临床分期和病理分级间差异具有显著性(P<0.05)。Spearman秩和相关检验提示存活素蛋白的表达水平与肿瘤的增值活性呈正相关(P<0.001),而与肿瘤细胞的AI无明显的相关性(P>0.005).结论:存活素蛋白表达在膀胱移行细胞癌的恶性进展中起重要作用,可作为评估膀胱移行细胞癌的恶性程度和预后的指标。
OBJECTIVE: To investigate the expression of survivin gene in transitional cell carcinoma of the urinary bladder and the relationship between the expression of survivin gene and the apoptosis and proliferation activity of cancer line. Methods: Western blot was used to detect the expression of Survivin protein in 128 cases of BTCC. The apoptosis index (AI) of the tumor cells was detected by DNA end-labeling method in situ. The Ki-67 monoclonal antibody was used to detect the tumor Cell proliferative activity (Ki-67 LI). Results: The results of Wstern blot analysis showed that Survivin was highly expressed in tumor tissue, while survivin protein was not expressed in normal bladder tissue. Survivin protein expression levels in the clinical stage and pathological grade difference was significant (P <0.05). Spearman rank correlation test indicated that survivin protein expression was positively correlated with tumor proliferation activity (P <0.001), but not with tumor AI (P> 0.005) .Conclusion: The expression of survivin in bladder It plays an important role in the malignant progression of transitional cell carcinoma and can be used as an index to evaluate the malignancy and prognosis of transitional cell carcinoma of the bladder.