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目的 :研究波形蛋白 (vimentin)在乳腺癌上皮中的表达及其与乳腺癌病理特征的关系。方法 :用免疫组化检测vimentin、增生细胞核抗原 (PCNA)、细胞角蛋白 (cytokeratin)等在乳腺癌上皮中的表达 ,用DNA斑点杂交检测c myc癌基因在乳腺癌组织中的表达 ,用葡聚糖包裹活性碳吸附法检测乳腺癌组织的雌激素受体 (ER)值。结果 :Vimentin可在 2 6 87%的乳腺癌组织中表达 ,其表达与乳腺癌的组织学分级、ER阴阳性和PCNA指数等一系列特征有关。Vimentin表达阳性的乳腺癌 ,其细胞形态与一般的乳腺癌上皮有所不同 ,细胞角蛋白阳性程度减弱 ,c myc癌基因较多发生扩增。结论 :Vimentin表达阳性的乳腺癌是一类恶性度较高、预后可能不良的乳腺癌。
Objective: To study the expression of vimentin in breast epithelium and its relationship with the pathological features of breast cancer. Methods: The expression of vimentin, proliferating cell nuclear antigen (PCNA) and cytokeratin in breast cancer tissues was detected by immunohistochemistry. The expression of c-myc oncogene in breast cancer tissues was detected by DNA dot blot. Detection of estrogen receptor (ER) in breast cancer by polysaccharides encapsulated activated carbon adsorption. Results: Vimentin was expressed in 2687% of breast cancer tissues. The expression of Vimentin correlated with histological grade, ER positive and PCNA index in breast cancer. Vimentin-positive breast cancer, the cell morphology and general breast epithelial different, decreased cytokeratin-positive, c myc oncogene more amplification. Conclusions: Vimentin-positive breast cancer is a type of malignant breast cancer with poor prognosis.