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醋酸格拉替雷是治疗多发性硬化症的一线药物,可通过诱导调节性CD8+T细胞发挥免疫调节作用。为研究GA诱导的CD8+T淋巴细胞,我们将茶树菇分离的两种凝集素AAL(Agrocybe aegerita lectin)和AAL2(Agrocybe aegerita lectin 2),通过生物素标记结合流式细胞仪,分别分析GA诱导的免疫耐受小鼠模型中脾脏、腹股沟淋巴结CD8+T淋巴细胞的糖基化水平,及细胞因子的表达。发现脾脏AAL+CD8+,AAL2+CD8+T淋巴细胞的比例(%)分别为8.46±3.1和9.08±2.8,淋巴结AAL+CD8+,AAL2+CD8+T淋巴细胞的比例(%)分别为10.65±4.6和8.25±5.9,且脾脏、淋巴结CD8+T淋巴细胞中AAL+细胞的百分比均显著高于CD8-T淋巴细胞(p<0.05)。淋巴结中AAL+CD8+T细胞表达IFN-γ的细胞比例显著高于AAL-CD8+T细胞,AAL2+CD8+T细胞表达IFN-γ和perforin细胞比例均显著高于AAL2-CD8+T细胞。该结果为CD8+T淋巴细胞表面糖基化研究提供了初步数据,并暗示糖基化水平与细胞因子分泌有关。
Glatiramer acetate is the first-line drug for the treatment of multiple sclerosis and can play an immunomodulatory role by inducing regulatory CD8 + T cells. In order to study GA-induced CD8 + T lymphocytes, we analyzed the induction of GA by Agrobybe aegerita lectin and AAL2 (Agrocybe aegerita lectin 2) separated by Agrocybe aegerita through biotin-labeled flow cytometry Glycosylation levels of CD8 + T lymphocytes in spleen and inguinal lymph nodes and expression of cytokines in a mouse model of immune tolerance. The percentages (%) of AAL + CD8 + and AAL2 + CD8 + T lymphocytes of spleen were 8.46 ± 3.1 and 9.08 ± 2.8, respectively. The percentages of lymphocytes AAL + CD8 + and AAL2 + CD8 + T lymphocytes were 10.65 ± 4.6 And 8.25 ± 5.9 respectively, and the percentages of AAL + cells in spleen and lymph node CD8 + T lymphocytes were significantly higher than that of CD8-T lymphocytes (p <0.05). The percentage of cells expressing IFN-γ in AAL + CD8 + T lymphocytes was significantly higher than that of AAL-CD8 + T cells in AAL2 + CD8 + T cells. The ratio of IFN- γ and perforin cells in AAL2 + CD8 + T cells was significantly higher than that in AAL2-CD8 + T cells. This result provides preliminary data on the surface glycosylation of CD8 + T lymphocytes and suggests that the level of glycosylation correlates with cytokine secretion.