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目的:以前期研究为依托,利用丹参EST-SSR分子标记法进一步构建丹参高密度遗传图谱,为丹参重要性状相关基因的定位、克隆及分子标记辅助选育新品种等研究提供技术基础。方法:利用紫花丹参74(母本)×白花丹参18(父本)两个品系杂交所得到的F1代植株,对411对引物进行PCR扩增及多态性筛选,结合前期研究得到的标记数据,利用Joinmap 4.0作图软件进行数据整合构建图谱。结果:用2个亲本共筛选了411对EST-SSR引物,共有328对引物有稳定的扩增产物,得到多态性引物164对。结论:最终构建了一张包含150个分子标记的高密度丹参遗传连锁图谱。
OBJECTIVE: To establish a high-density genetic map of Salvia miltiorrhiza by EST-SSR molecular marker method based on previous research, and to provide a technical basis for the research on the location, cloning and molecular marker-assisted breeding of Salvia miltiorrhiza. Methods: F1 plants of F1 hybrid between Salvia miltiorrhiza 74 (female) × Salvia miltiorrhiza Bunge 18 (male) were screened, and 411 pairs of primers were amplified by PCR and selected for polymorphism. Combined with the data from the previous study , Using Joinmap 4.0 mapping software for data integration to build the map. Results: A total of 411 pairs of EST-SSR primers were screened from 2 parents. A total of 328 pairs of primers had stable amplification products, and 164 pairs of polymorphic primers were obtained. Conclusion: A high molecular linkage map of Salvia miltiorrhiza with 150 molecular markers was constructed.