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目的:探讨IL-13在哮喘患儿血清中的表达及槐杞黄对于哮喘大鼠IL-13表达的可能影响及与布地奈德的共同作用。方法:选择10例哮喘缓解期儿童(哮喘组)及10例健康儿童(健康对照组)为研究对象。两组年龄、性别、体质指数比较差异均无统计学意义。测试两组儿童初诊时肺功能,并利用酶联免疫吸附试验(enzymelinkedimmunosorbentassay,ELISA)检测两组儿童血清IL-13的水平。取50只4~6周龄的健康雄性SD大鼠,适应性喂养1周后,随机分为5组,即对照组、哮喘模型组、布地奈德组、槐杞黄组、布地奈德联合槐杞黄组。用卵清白蛋白(ovalbumin,OVA)致敏及激发共持续6周,建立哮喘模型。通过HE染色观察肺部炎症细胞浸润情况。应用ELISA检测及比较各组大鼠之间肺泡灌洗液(bronchoalveolar lavage fluid,BALF)及血清中IL-13浓度的差异。结果:与健康对照组儿童比较,哮喘组儿童初诊时第1秒用力呼气量占预测值百分比及呼气峰流速占预测值百分比均升高,血清IL-13水平升高(P<0.05)。大鼠肺组织病理学改变:模型组气道周围大量炎症细胞浸润、柱状细胞增生、气管壁增厚;对照组则无上述表现;相比模型组,布地奈德组、槐杞黄组、布地奈德联合槐杞黄组大鼠不同程度减轻(P<0.05)。模型组BALF及血清中IL-13的浓度增加(P<0.05)。相较于模型组,布地奈德组、槐杞黄组、布地奈德联合槐杞黄组大鼠BALF及血清IL-13浓度有不同程度降低(P<0.05),且布地奈德联合槐杞黄组变化比布地奈德组更明显(P<0.05)。结论:在OVA诱导的大鼠哮喘模型中,使用布地奈德、槐杞黄或是布地奈德联合槐杞黄治疗可以不同程度改善气道IL-13分泌,因而可能改善气道炎症。布地奈德联合槐杞黄治疗可能存在协同效应。
Objective: To investigate the expression of IL-13 in serum of asthmatic children and the possible effect of Huai Qi Huang on the expression of IL-13 in asthmatic rats and its interaction with budesonide. Methods: Ten children with asthma (asthma group) and 10 healthy children (healthy control group) were selected as the study subjects. There was no significant difference between the two groups in age, gender and body mass index. The lung function of the two groups of newborns at the time of first visit was tested. The levels of IL-13 in serum of the two groups were detected by enzyme linked immunosorbentassay (ELISA). Fifty healthy male SD rats aged 4 ~ 6 weeks were randomly divided into five groups: control group, asthma model group, budesonide group, Huai Qi Huang group, budesonide group Huaiqi yellow group. Allergen and challenge with ovalbumin (OVA) for 6 weeks to establish an asthma model. Infiltration of inflammatory cells in the lungs was observed by HE staining. The levels of IL-13 in bronchoalveolar lavage fluid (BALF) and serum in rats of different groups were detected by ELISA. Results: Compared with children in healthy control group, the percentage of predictive value of forced expiratory volume at 1 second and the percentage of predicted peak expiratory flow rate in asthma group were significantly higher than those in healthy control group (P <0.05) . Pathological changes of lung tissue in rats: a large number of inflammatory cells around the airway infiltration, columnar cell hyperplasia, tracheal wall thickening; the control group did not show the above; compared with the model group, budesonide group, Huai Qi Huang group, Budi Ned and Huaiqi group were relieved to varying degrees (P <0.05). The concentration of IL-13 in BALF and serum in the model group increased (P <0.05). Compared with the model group, the concentration of BALF and serum IL-13 in the budesonide group, the Huaiqi group, the budesonide group and the Huaiqi group were decreased to some extent (P <0.05), and budesonide combined with Huaiqi The change of yellow group was more obvious than budesonide group (P <0.05). CONCLUSIONS: In the OVA-induced rat asthma model, administration of budesonide, Huai Qi Huang or budesonide combined with Huai Qi Huang can improve airway IL-13 secretion to varying degrees and thus may improve airway inflammation. Budesonide combined Huai Qi Huang treatment may exist synergistic effects.