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目的分析早期肺癌患者和肺良性病变患者主气道上皮细胞表皮生长因子受体(EGFR)mRNA表达情况,探讨其在早期肺癌诊断中的价值。方法采用实时荧光定量聚合酶链反应(real-time PCR)方法检测33例肺癌患者和70例肺良性疾病患者主气道上皮细胞EGFR mRNA表达。结果早期肺癌患者主气道上皮EGFR mRNA的平均表达水平为肺良性疾病患者平均表达水平的4.89倍,两者差异具有统计学意义(P<0.05);且早期肺癌患者主气道非病变部位EGFR mRNA表达与病变部位EGFR mRNA表达无明显差异。结论早期肺癌患者主气道上皮EGFR mRNA表达明显高于肺良性疾病。
Objective To analyze the expression of epidermal growth factor receptor (EGFR) mRNA in primary airway epithelial cells in patients with early lung cancer and benign lung diseases and to explore its value in the diagnosis of early lung cancer. Methods Real-time quantitative PCR (real-time PCR) was used to detect the expression of EGFR mRNA in the airway epithelium of 33 patients with lung cancer and 70 patients with benign lung disease. Results The average expression level of EGFR mRNA in primary airway epithelium of patients with early lung cancer was 4.89 times that of patients with benign lung disease, the difference was statistically significant (P <0.05) There was no significant difference in the expression of EGFR mRNA between the mRNA and the lesion site. Conclusion The expression of EGFR mRNA in primary airway epithelium of patients with early lung cancer is significantly higher than that of benign lung disease.