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人类抑癌基因beclin 1通过自噬作用调节细胞生长,但在胃癌和直结肠癌中其表达水平和调控机制仍不清楚.通过检测胃癌和直结肠肿瘤组织中beclin 1基因的表达水平,及DNA异常甲基化和杂合子缺失对其表达的影响,发现与癌旁组织相比,35%的胃癌标本和30%的直结肠癌标本中beclin 1基因表达显著下调.同时发现,beclin 1基因5′端存在一高密度CpG岛,在胃癌和直结肠癌中beclin 1的启动子区域和第二个内含子区域存在甲基化,而杂合子缺失仅在胃癌中发生.这些发现表明beclin 1基因的异常甲基化和杂合子缺失对其在胃癌和直结肠癌中的表达起调控作用.
The expression of beclin 1, a regulator of human tumor suppressor gene, regulates cell growth by autophagy, but its expression and regulation in gastric cancer and in colorectal cancer remain unclear. By detecting the expression of beclin 1 in gastric cancer and in colorectal cancer, Abnormal methylation and heterozygosity deletions on its expression and found that compared with adjacent tissues, 35% of gastric cancer specimens and 30% of colorectal cancer specimens beclin 1 gene expression was significantly reduced.At the same time, beclin 1 gene 5 There is a high-density CpG island with methylation of the promoter region and the second intron region of beclin 1 in gastric and colorectal cancers, whereas loss of heterozygotes only occurs in gastric carcinomas These findings suggest that beclin 1 Aberrant methylation of genes and loss of heterozygotes play a regulatory role in gastric cancer and colorectal cancer.