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作者从Tel Aviv医疗中心的HIV免疫学诊所的门诊病人中挑选了20名男性同性恋者,其中3人是HIV血清阴性,其余17人为HIV血清阳性(年龄从19~50岁,平均为30岁。)所有受试者均皮下注射1.0ml脊髓灰质炎灭活疫苗(IPV)。于注射疫苗前和注射疫苗后4个星期采血以测定血清中的抗体水平。将前后采集的血清配成对同时做4倍稀释培养20小时进行脊髓灰质炎中和抗体试验。应用leu4、leu3、leu2的单克隆抗体对淋巴细胞进行评估,并通过血细胞计数进行分析。用植物血凝素(PHA)、美洲商陆
The authors selected 20 male homosexuals from outpatients at the HIV Immunology Clinic at Tel Aviv Medical Center, three of whom were HIV seronegative and the remaining 17 were HIV seropositive (age 19 to 50 years with an average of 30 years). ) All subjects were injected subcutaneously with 1.0 ml poliomyelitis inactivated vaccine (IPV). Blood was taken 4 weeks prior to and after vaccination to determine the level of antibody in serum. Serum collected before and after dubbed 4 times diluted at the same time for 20 hours for polio neutralization antibody test. Lymphocytes were assessed using monoclonal antibodies to leu4, leu3 and leu2 and analyzed by hematocrit. Phytohemagglutinin (PHA), Pokeweed