论文部分内容阅读
材料和方法试材取于佛罗里达州中部研究教育中心,里斯本葡萄试验园的七年生葡萄.在葡萄开花前立即去雄,用新鲜的或贮藏在-14℃下的花粉授粉,具体作法为:先将花粉放在小瓶中,用玻棒粘取花粉点在柱头上.去雄和授粉的穗子套上纸袋,以防接受外来花粉.在授粉后分别10、20、30、40、60天时采回果穗,用含1滴表面活性剂的1.3%NaCIO进行表面消毒5分钟,再用无菌去离子水冲冼2次.
MATERIALS AND METHODS Specimens were taken from a seven-year-old grapes plant at the Central Florida Research and Education Center in Lisbon, Portugal. The grapes were exhumed immediately prior to flowering and pollinated with fresh or stored pollen at -14 ° C: Place the pollen in a vial and stick the pollen point on the stigma with a glass stick.Expand and pollinate the ear with a paper bag to prevent the foreign pollen from being harvested.Early ears are recovered at 10, 20, 30, 40 and 60 days after pollination , Surface-sterilized with 1.3% NaCIO with 1 drop of surfactant for 5 minutes and rinsed twice with sterile deionized water.