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目的：研究人骨髓瘤细胞的ＩＬ－６信号转导通路中哪一个环节可作为“ＩＬ－６功能拮抗剂”设计的目标靶位。方法：首先通过凝胶阻滞电泳（ｅｌｅｃｔｒｏｐｈｏｒｅｔｉｃ ｍｏｂｉｌｉｔｙ ｓｈｉｆｔ ａｓｓａｙ，ＥＭＳＡ）、免疫沉淀方法观察人骨髓瘤细胞Ｓｋｏ－００７中参与ＩＬ－６信号转导功能的转录因子－ＳＹＡＴ３，ＮＦ－ＩＬ－６和蛋白做酶ＥＲＫ的诱导活化情况，继而构建和设计了针对这些信号分子的反义表达质粒和反义寡核苷酸，用同样方法观察反义核酸对ＩＬ－６信号转导功能的影响。结果：这些反义核酸可以不同程度地抑制ＩＬ－６在Ｓｋｏ－００７细胞中的信号转导功能。结论：可通过进一步实验确定转录因子、蛋白激酶作为“胞内拮抗剂”作用目标靶位的可行性。
Objective: To investigate which part of IL-6 signal transduction pathway in human myeloma cells can be used as a target of “IL-6 functional antagonist” design. Methods: The transcription factors-SYY3, NF-IL-6 and IL-6 involved in IL-6 signaling in human myeloma cell line Sko-007 were first observed by electrophoretic mobility shift assay (EMSA) Then the antisense expression plasmids and antisense oligonucleotides targeting these signal molecules were constructed and designed. The influence of antisense nucleic acids on IL-6 signal transduction function was observed in the same way. Results: These antisense oligonucleotides inhibited the signal transduction function of IL-6 in Sko-007 cells to varying degrees. CONCLUSIONS: The viability of target targets for transcriptional factors and protein kinases as “intracellular antagonists” can be determined by further experimentation.