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目的观察半叶马尾藻多糖(Sargassum hemiphyllum polysac charides,SHP)口服液对辐射损伤小鼠的抗辐射作用及对其血清自由基的影响。方法 50只昆明系小鼠随机分为正常对照组、辐射对照组、低剂量SHP组、中剂量SHP组、高剂量SHP组各10只,正常对照组、辐射对照组应用1mL生理盐水灌胃,低、中、高剂量SHP组分别应用100、200、300mg/kg的SHP溶液1mL灌胃,连续14d。辐射对照组和低、中、高剂量SHP组于第15天一次性腹腔注射碘化钠溶液0.5mL诱发辐射损伤,24h后检测各组胸腺指数和脾脏指数及血清白细胞介素-2(interleukin-2,IL-2)、超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)和丙二醛(malondialdehyde,MDA)水平,观察刀豆球蛋白A(concanavalin A,ConA)诱导的脾细胞和胸腺细胞增殖反应。结果正常对照组胸腺指数、脾脏指数、血清IL-2、SOD、CAT、MDA及ConA诱导的脾脏细胞和胸腺细胞增殖分别为(32.12±5.88)×10-4、(53.56±6.25)×10-4、(37.56±6.21)ng/L、(350.76±31.34)u/mL、(2.53±0.68)u/mL、(5.86±1.25)μmol/L、1.618±0.560、1.332±0.450,辐射对照组分别为(11.21±3.37)×10-4、(30.24±5.13)×10-4、(18.08±3.24)ng/L、(238.48±17.13)u/mL、(0.95±0.26)u/mL、(10.33±1.37)μmol/L、0.562±0.220、0.438±0.180,低剂量SHP组分别为(29.37±3.34)×10-4、(61.42±8.14)×10-4、(30.68±5.05)ng/L、(311.72±23.14)u/mL、(1.91±0.32)u/mL、(6.92±1.34)μmol/L、0.926±0.360、0.823±0.250,中剂量SHP组分别为(33.52±5.14)×10-4、(72.28±5.56)×10-4、(34.62±5.71)ng/L、(312.97±35.56)u/mL、(1.92±0.48)u/mL、(6.78±1.14)μmol/L、0.914±0.320、0.836±0.310,高剂量SHP组分别为(45.78±6.18)×10-4、(76.28±6.67)×10-4、(35.89±6.15)ng/L、(319.76±26.67)u/mL、(2.02±0.35)u/mL、(6.56±1.18)μmol/L、0.936±0.480、1.082±0.520,正常对照组和低、中、高剂量SHP组与辐射对照组比较差异均有统计学意义(P<0.05),且随SHP剂量增高,血清自由基水平增高,辐射损伤得到改善(P<0.05)。结论 SHP口服液可提高辐射损伤小鼠的抗氧化酶活性、抑制脂质过氧化,抗辐射作用与SHP剂量呈正相关。
Objective To investigate the anti-radiation effects of Sargassum hemiphyllum polysac charides (SHP) oral solution on serum free radicals in radiation-injured mice. Methods Fifty Kunming mice were randomly divided into normal control group, radiation control group, low-dose SHP group, middle-dose SHP group and high-dose SHP group, 10 normal control group and radiation control group, Low, medium and high doses of SHP group were 100,200,300 mg / kg SHP solution 1mL intragastric administration, continuous 14d. Radiation control group and low, medium and high dose SHP group were given intraperitoneal injection of 0.5mL sodium iodide solution on day 15 to induce radiation injury. Thymus index, spleen index and serum levels of interleukin- 2, IL-2, superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) were measured. Concanavalin A ) Induced splenocyte and thymocyte proliferation responses. Results Thymic index, spleen index, serum IL-2, SOD, CAT, MDA and ConA in normal control group were (32.12 ± 5.88) × 10-4 and (53.56 ± 6.25) × 10- (37.56 ± 6.21) ng / L, (350.76 ± 31.34) u / mL, (2.53 ± 0.68) u / mL, (5.86 ± 1.25) μmol / L and 1.618 ± 0.560,1.332 ± 0.450 Were (11.21 ± 3.37) × 10-4, (30.24 ± 5.13) × 10-4, (18.08 ± 3.24) ng / L, (238.48 ± 17.13) u / mL, ± 1.37 μmol / L, 0.562 ± 0.220 and 0.438 ± 0.180, respectively, and the low dose SHP group was (29.37 ± 3.34) × 10-4, (61.42 ± 8.14) × 10-4 and (30.68 ± 5.05) ng / L, (311.72 ± 23.14) u / mL, (1.91 ± 0.32) u / mL, (6.92 ± 1.34) μmol / L, 0.926 ± 0.360 and 0.823 ± 0.250 respectively, and middle dose SHP group was (33.52 ± 5.14) × 10-4 , (72.28 ± 5.56) × 10-4, (34.62 ± 5.71) ng / L, (312.97 ± 35.56) u / mL, (1.92 ± 0.48) u / mL, (6.78 ± 1.14) μmol / L, 0.914 ± 0.320 , 0.836 ± 0.310 and (45.78 ± 6.18) × 10-4, (76.28 ± 6.67) × 10-4, (35.89 ± 6.15) ng / L, (319.76 ± 26.67) u / mL, 2.02 ± 0.35) u / mL, (6.56 ± 1.18) μmol / L, 0.936 ± 0.480, 1.08 2 ± 0.520. There was significant difference between normal control group and low, medium and high dose SHP group and radiation control group (P <0.05). With the increase of SHP dose, serum free radical level increased and radiation damage was improved P <0.05). Conclusion SHP oral solution can increase antioxidant enzyme activity and inhibit lipid peroxidation in radiation-injured mice, and the anti-radiation effect is positively correlated with SHP dose.