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细胞培养液是精子体外处理的常用介质。本研究通过精子在不同培养介质中进行上游孵育处理，并采用精液自动分析仪（ＣＡＳＡ）观察分析。结果显示：Ｔｙｒｏｄｅ’ｓ液、Ｔ６液及ＨＴＦ液可显著提高精子前向运动率和前向运动速度，ＢＷＷ液改变不明显。培养液相互之间比较提示ＨＴＦ液还可获得较佳的精子轨迹速度、精子头部摆动频率、摆动振幅等，且上游回收率明显高于其它介质。并就培养液成分、配制特点对精子、人工授精的影响进行了探讨。
Cell culture medium is a common medium for sperm in vitro processing. In this study, spermatozoa were incubated in different culture mediums upstream, and analyzed by CASA. The results showed that: Tyrode’s liquid, T6 liquid and HTF liquid can significantly increase the sperm forward movement rate and forward movement speed, BWW liquid change was not obvious. The comparison between the culture solutions suggests that the HTF solution can also obtain better sperm trajectory speed, sperm head swing frequency and swing amplitude, and the recovery rate of the upstream is obviously higher than that of other media. The effects of culture fluid composition and preparation on sperm and artificial insemination were also discussed.