Effects of clonidine on myocardial β-adrenergic receptor-adenyl cyclase-cAMP system after scalds in

• 作 者: 何华美 孙继武 肖成荣 宋裕南
• 机 构: 第三军医大学药理教研室
• 出 处: Acta Pharmacologica Sinica
• 发表日期: 1997年02期
• 关键词: cyclase after clonidine Effects in myocardial of on rats scalds

研究可乐定（Ｃｌｏ）对大鼠烫伤后心脏β肾上腺素受体（βＡＲ）腺苷酸环化酶（ＡＣ）环腺苷一磷酸（ｃＡＭＰ）系统的作用．方法：大鼠于９５℃水浴中烫９ｓ，造成背部３０％皮肤全层烫伤．用放射受体分析、间接方法、酶放射化学分析、放射免疫分析分别测定βＡＲ密度和亲和力，ＡＣ和磷酸二酯酶（ＰＤＨ）活性及ｃＡＭＰ生成量．结果：Ｃｌｏ（０３、１０和３０ｍｇ·ｋｇ－１）增加烫伤后１２ｈ心肌βＡＲ密度、ＡＣ活性及ｃＡＭＰ生成量（Ｐ＜００５或００１）；但Ｃｌｏ对烫伤大鼠心肌βＡＲ亲和力、ＰＤＨ活性及正常大鼠上述各项指标均无影响（Ｐ＞００５）．育亨宾００５ｍｇ·ｋｇ－１，部分逆转Ｃｌｏ对βＡＲ密度、ＡＣ活性和ｃＡＭＰ生成的作用；哌唑嗪００３ｍｇ·ｋｇ－１，对Ｃｌｏ的作用无明显影响．结论：Ｃｌｏ抑制烫伤后大鼠心肌βＡＲＡＣｃＡＭＰ系统的变化．

To study the effect of Clo on cardiac β-adrenergic receptor (AR) -acyl-cyclic adenosine monophosphate (cAMP) system in rats after scald. METHODS: Rats were hot 9s in a 95 ° C water bath, causing 30% of the skin’s entire skin to burn on the back. The density and affinity of βAR, the activity of AC and phosphodiesterase (PDH) and the amount of cAMP production were measured by radioimmunoassay, indirect method, enzymatic radiochemical analysis and radioimmunoassay. Results: Clo (03,10 and 30mg · kg-1) increased myocardial βAR density, AC activity and cAMP production (P <005 or 001) at 12h after burn injury. However, Clo Β-AR affinity of myocardium in scalded rats, PDH activity and normal rats had no effect on the above indicators (P> 0  05). Yohimbine 0  05mg · kg-1, partial reversal of Clo on β-AR density, AC activity and cAMP production; prazosin 0  03mg · kg-1, no significant effect on the role of Clo. Conclusion: Clo inhibits the changes of βARACcAMP system in rat myocardium after scald.