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目的对2011年8月采集的从老挝输入的登革热病人急性期血清进行登革病毒分离,以找出病原学证据。方法用C6/36白纹伊蚊细胞进行病毒分离,对有细胞病变的标本进行RT-PCR,检测登革病毒RNA及型别,对PCR产物测序,进行比对分析和进化树分析。结果从8例输入登革热病人的血清中分离到4株能引起细胞病变的病毒,均为登革病毒1型,与泰国毒株的同源性>99%。结论云南省首次从输入登革热病人血清中分离到登革病毒1型,为云南省登革热的防控提供了依据。
Objective To isolate dengue virus from the acute phase of dengue fever patients collected from Laos in August 2011 to find out the etiological evidence. Methods C6 / 36 Aedes albopictus cells were used for virus isolation, RT-PCR was performed on the samples with cytopathic effect, the dengue virus RNAs and their genotypes were detected, and the PCR products were sequenced and analyzed by comparison and phylogenetic tree analysis. Results Four isolates of dengue virus were isolated from the serum of 8 imported dengue patients. All of them were dengue virus type 1, which shared 99% identity with that of the Thai strain. Conclusions Yunnan dengue virus type 1 was first isolated from the serum of imported dengue patients, which provided a basis for prevention and control of dengue fever in Yunnan Province.