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目的:观察间歇性低氧-复氧对少突胶质前体细胞增殖的影响。方法:取分离纯化的少突胶质前体细胞,培养皿或96孔板中的细胞分别加入10mg/ml的各种细胞因子,分为常氧组和低氧组,低氧组在3%低氧环境下持续4d分别各培养1h、2h、3h、4h后取出复氧,9d后进行MTT测定或细胞计数。96孔板分组;无细胞的阴性对照,有细胞的空白对照,PDGF,bFGF,IL-6,IFN-β1,GDNF,PDGF+bFGF,PDGF+IL-6,PDGF+GDNF,bFGF+IL- 6,bFGF+GDNF,IL-6+GDNF,PDGF+bFGF+IL-6,PDGF+bFGF+GDNF,PDGF+bFGF+IL-6+GDNF共16组。培养皿分组除无细胞的阴性对照组外的15组。培养至9d。同时取出各组细胞,进行细胞计数和MTT测定。结果:除1h组外,各低氧处理组细胞数及MTT值均较对照明显增多。结论:首次报道恰当时间的间歇性低氧-复氧可促进少突前体细胞增殖。这为细胞移植治疗中枢神经系统脱髓鞘疾病及研究髓鞘形成、髓鞘再生的机理提供基础。
Objective: To observe the effect of intermittent hypoxia-reoxygenation on the proliferation of oligodendrocyte precursor cells. Methods: Purified oligodendrocyte precursor cells were obtained. The cells in culture dish or 96-well plate were added with 10mg / ml of various cytokines and divided into normoxia group and hypoxia group. The hypoxia group was treated with 3% Under hypoxia for 4 days, the cells were incubated for 1h, 2h, 3h and 4h respectively, and then reoxygenated. After 9 days, MTT assay or cell count was performed. 96-well plate, cell-free negative control, blank control with cells, PDGF, bFGF, IL-6, IFN-β1, GDNF, PDGF + bFGF, PDGF + IL-6, PDGF + GDNF, bFGF + IL- 6 , bFGF + GDNF, IL-6 + GDNF, PDGF + bFGF + IL-6, PDGF + bFGF + GDNF, PDGF + bFGF + IL-6 + GDNF. Petri dishes divided into 15 groups except the cell-free negative control group. Cultivation to 9d. At the same time, each group of cells was removed for cell counting and MTT assay. Results: In addition to 1h group, the number of cells and MTT in each hypoxia group were significantly increased compared with the control. CONCLUSIONS: Intermittent hypoxia-reoxygenation, which was first reported at the appropriate time, promotes oligodendrocyte precursor cell proliferation. This provides a basis for cell transplantation in the treatment of demyelinating diseases of the central nervous system and the mechanism of myelination and remyelination.