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目的:研究CO2人工气腹对裸鼠腹腔宫颈癌细胞生长与转移的影响。方法:建立宫颈癌裸鼠模型,随机分为气腹组、开腹组和对照组,麻醉后分组进行实验,4周后处死全部裸鼠,打开腹腔,依次查找腹腔器官,分离肿瘤组织,计数各脏器肿瘤转移数,称量转移瘤质量,同时检测裸鼠术前术后体质量下降情况。结果:CO2气腹组、开腹组和对照组术后4周腹腔生长的肿瘤质量分别为(1.79±0.04)g、(0.84±0.29)g和(0.83±0.35)g;与开腹组和对照组比较,CO2气腹组明显增加,P<0.01,开腹组与对照组比较差异无统计学意义,P>0.05。气腹组腹壁切口转移瘤较大,腹腔种植和播散较严重。实验后开腹组裸鼠体质量下降显著大于人工气腹组和对照组,P<0.05。结论:CO2人工气腹较开腹手术更易促进腹腔肿瘤播散和种植转移。
Objective: To study the effect of CO2 artificial pneumoperitoneum on the growth and metastasis of celiac cervical carcinoma in nude mice. Methods: The nude mice model of cervical cancer was established and randomly divided into pneumoperitoneum group, laparotomy group and control group. After anesthesia, the rats were divided into groups for experiment. After 4 weeks, all the nude mice were sacrificed, the abdominal cavity was opened, the abdominal organs were sequentially examined, tumor tissue was separated and counted The number of tumor metastasis of each organ, weighed the mass of metastatic tumor, meanwhile, the decline of body mass of nude mice before and after operation was detected. Results: The tumor mass in peritoneal cavity of CO2 pneumoperitoneum group, open group and control group at 4 weeks after operation were (1.79 ± 0.04) g, (0.84 ± 0.29) g and (0.83 ± 0.35) g, respectively. Compared with the control group, CO2 pneumoperitoneum group increased significantly, P <0.01, there was no significant difference between the open group and the control group (P> 0.05). Gas pneumoperitoneum abdominal wall incision metastases larger, more serious abdominal cultivation and dissemination. After the experiment, the body weight of the nude mice in the open group was significantly lower than that in the artificial pneumoperitoneum group and the control group (P <0.05). CONCLUSION: CO2 pneumoperitoneum is more likely to promote peritoneal tumor dissemination and metastasis than laparotomy.