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目的比较经腹腔注射与经尾静脉注射骨髓间充质干细胞治疗大鼠白毒伞中毒致急性肝衰竭的疗效。方法选取10只体重约80 g的雄性清洁级SD大鼠用于骨髓间充质干细胞(BMSCs)培养。另选取体重220~250 g的成年大鼠60只作为研究对象,用于肝衰竭模型制备以及BMSCs输注治疗。将其中15只大鼠作为空白对照组,剩下的45只均给予白毒伞粗毒素灌胃制备肝衰竭模型,将造模成功的大鼠随机均分为3组,即肝衰竭模型组、经腹腔注射治疗组及经尾静脉注射治疗组。对两治疗组予BMSCs经两种途径输注治疗,对比各组大鼠7 d生存率及肝功能、凝血功能、炎症因子等指标情况。结果与肝衰竭模型组相比,两治疗组SD大鼠体重减轻明显更少(P<0.05),7 d生存率要明显更高[73.3%、80.0%VS 20.0%](P<0.05),两治疗组上述指标比较差异无统计学意义(P>0.05)。治疗后两治疗组大鼠肝功能、凝血功能、炎症因子等指标均较肝衰竭模型组显著改善(P<0.05),两治疗组上述指标比较差异无统计学意义(P>0.05),空白对照组大鼠肝功能、凝血功能、炎症因子等指标与其他三组比较,差异均有统计学意义(P<0.05)。结论经腹腔注射和经尾静脉注射两种输注途径骨髓间充质干细胞对白毒伞中毒致急性肝衰竭大鼠具有十分明显的治疗作用,能显著改善大鼠肝功能衰竭,且效果相当。
Objective To compare the therapeutic effects of intraperitoneal injection of mesenchymal stem cells via caudal vein on acute liver failure induced by toxin in rats. Methods Ten male SD rats weighing 80 g were used in the culture of BMSCs. In addition, 60 adult rats weighing 220-250 g were selected as the research object for the preparation of liver failure model and BMSCs infusion therapy. Fifteen of them were used as blank control group. The remaining 45 rats were given aphrodisiacal venom for intragastric administration of hepatic failure model. Rats with successful model were randomly divided into three groups: model group with liver failure, After intraperitoneal injection and tail vein injection treatment group. The two treatment groups were given BMSCs by two ways of infusion therapy, compared 7 d survival rate of rats and liver function, coagulation, inflammation and other indicators. Results Compared with the liver failure model group, the SD rats in the two treatment groups had significantly less weight loss (P <0.05) and the survival rate at 7 d was significantly higher (73.3%, 80.0% vs 20.0%, P <0.05) There was no significant difference between the two treatment groups (P> 0.05). After treatment, the liver function, coagulation function, inflammatory factors and other indexes in the two treatment groups were significantly improved compared with those in the liver failure model group (P <0.05). There was no significant difference between the two treatment groups (P> 0.05) Compared with the other three groups, the indexes of liver function, coagulation function and inflammatory factors of the rats in the two groups were significantly different (P <0.05). Conclusion Intraperitoneal injection and tail vein injection of two kinds of infusion routes of bone marrow mesenchymal stem cells on white poisoned umbrella acute liver failure rats have a very significant therapeutic effect, can significantly improve liver failure in rats, and the effect is quite.