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目的 探讨肿瘤坏死因 β(TNF β)对培养的胰腺癌细胞体外诱导凋亡作用 ,阐明胰腺癌细胞死亡的规律和途径。方法 采用末端脱氧核苷转移酶凋亡原位染色方法检测肿瘤细胞凋亡。结果 不同浓度TNF β对人JF3 0 5胰腺癌细胞有凋亡诱导作用 ,且呈剂量依赖性 ,凋亡原位染色阳性细胞显示细胞核出现棕黄色颗粒。结论 TNF β抗肿瘤作用的部分机制与体外诱导人JF3 0 5胰腺癌细胞凋亡有关。
Objective To investigate the effect of tumor necrosis factor β (TNF β) on the induction of apoptosis in cultured pancreatic cancer cells in vitro and to elucidate the rules and pathways of pancreatic cancer cell death. Methods Apoptosis of tumor cells was detected by in situ dTSP staining. Results Different concentrations of TNFβ induced apoptosis of human JF3 05 pancreatic cancer cells in a dose-dependent manner. Apoptotic cells in situ showed brownish yellow granules in the nucleus. Conclusion The mechanism of anti-tumor effect of TNF-β is related to the apoptosis of human pancreatic cancer cell line JF-05 in vitro.