论文部分内容阅读
目的研究富含抗性淀粉转基因大米对大鼠肠道健康的影响。方法48只健康成年雄性SD大鼠按体重随机分为4组:分别给予非转基因大米最大量掺入的饲料(No-GM组)、转基因大米最大量和半量掺入的饲料(GM组、Half-GM组)和正常对照饲料重(ND组)。喂养5w后连续收集4d粪便;6w后处死大鼠并收集盲肠、结肠内容物,测定内容物和肠壁量、pH值、短链脂肪酸(SCFA)等反映肠道健康的指标。结果GM组的体重接近ND组,显著低于No-GM组。与No-GM组和ND组相比,GM组和Half-GM组粪便量、粪便水分、盲肠壁以及内容物含量显著增加,并存在显著的量效关系(P<0.05)。GM组结肠壁质量与No-GM组和ND组也有显著差异。盲肠、结肠和粪便中SCFA的含量逐渐降低,除了Half-GM组结肠中丁酸含量与其他各组没有差异外,GM组和Half-GM组盲肠、结肠中SCFA含量与No-GM组和ND组相比都有增加,各组大鼠粪便中丁酸含量的差异消失,但是乙酸和丙酸含量仍然存在差异。GM组和Half-GM组粪便和盲肠的pH值非常显著低于No-GM组和ND组。结论抗性淀粉转基因大米能改善大鼠肠道健康。
Aim To study the effect of transgenic rice rich in resistant starch on intestinal health in rats. Methods Forty-eight healthy adult male Sprague-Dawley rats were randomly divided into 4 groups according to their body weight: the highest amount of non-GM rice (No-GM group), the largest amount of GM rice and the half amount of GM -GM group) and normal control diet (ND group). The rats were sacrificed after 6 weeks of feeding, and the cecum and colon contents were collected. The contents of intestinal contents, intestinal volume, pH value and short-chain fatty acids (SCFA) were measured to reflect the gut health. Results The weight of GM group was close to that of ND group and significantly lower than that of No-GM group. Compared with No-GM group and ND group, the fecal volume, fecal water, cecum wall and contents of GM-treated and half-GM-treated rats significantly increased (P <0.05). There was also a significant difference in the quality of colon wall between GM group and No-GM group and ND group. The content of SCFA in cecum, colon and excrement decreased gradually. The contents of butyric acid in the colon of half-GM group were the same as those in other groups, Compared with the control group, the differences of butyric acid content in the feces of rats in each group disappeared, but the content of acetic acid and propionic acid still differed. The pH values of feces and cecum in GM and Half-GM groups were significantly lower than those in No-GM and ND groups. Conclusion Resistant starch transgenic rice can improve intestinal health in rats.