论文部分内容阅读
目的:研究日服用次数对大黄含药血清体外抗氧化效应的影响,制定大黄用于抗氧化的合理日服用次数。方法:Wistar大鼠日服1次组(G1),2次组(G2),3次组(G3),分别每日灌服大黄提取物3.6 g.kg-11次、1.8 g.kg-12次、1.2 g.kg-13次,分别于第1次给药前(0 h)及给药后0.083,0.167,0.25,0.5,1,2,4,6,8,10,12,24 h眼眶后静脉取血,用于测定大黄含药血清体外对超氧阴离子自由基(O2.)、二苯代苦味酰基自由基(DPPH)以及羟基自由基的清除抗氧化实验,并对其进行房室模型的拟合和参数计算,得到抗氧化效应-时间曲线(E-T曲线),制定大黄用于抗氧化时合理的日服用次数。结果:日服用次数与大黄抗氧化作用的强弱显著相关,给药2次E-T曲线下面积(AUCE)明显高于给药1次和3次(P<0.05)。结论:大黄用于抗氧化日服用次数以每日2次为宜。
OBJECTIVE: To study the effect of daily administration on the anti-oxidation effect of rhubarb serum and to establish the rational daily dose of rhubarb for anti-oxidation. Methods: Wistar rats were administered with G1, G2 and G3 on a daily basis, and rhubarb extract 3.6g.kg-11 times and 1.8g.kg-12 Times, 1.2 g.kg-13 times, respectively, before the first administration (0 h) and after administration 0.083,0.167,0.25,0.5,1,2,4,6,8,10,12,24 h Blood was collected from the retro-orbital vein for the determination of rhubarb serum containing antioxidant superoxide anion radical (O2.), Dibenzoyl acyl free radical (DPPH) and hydroxyl radical scavenging antioxidant experiments, and its room Room model fitting and parameter calculation, anti-oxidation effect - time curve (ET curve), the development of rhubarb for anti-oxidant reasonable number of daily doses. Results: The number of daily administration was significantly correlated with the antioxidant capacity of rhubarb. The area under the curve of E-T twice administration (AUCE) was significantly higher than that of administration once and three times (P <0.05). Conclusion: rhubarb for anti-oxidant daily times to 2 times a day is appropriate.