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To investigate the effect of nano-apatite on the expression of the telomerase gene of human hepatocellular carcinoma cell lines and further explore the mechanism of the nano-apatite inhibiting cancer cells. Using the hybridization in situ method to detect the expression of the telomerase gene of human hepatocellular carcinoma cells treated with the nano-apatite for 4 h at 37 ℃. The hybridization in situ showed that the cytoplasm of the positive cells was stained in nigger-brown. The positive cell rate of the control group was 88.49%, the cisplatin group was 25.6%, the nano-apatite group was 63.6%. The activity of telomerase gene was both obviously declined comparing with the control group and the difference had significance (p<0.05, p<0.01). The nano-apatite obviously inhabit the expression of the telomerase gene of human hepatocellular carcinoma cells.
To investigate the effect of nano-apatite on the expression of the telomerase gene of human hepatocellular carcinoma cell lines and further explore the mechanism of the nano-apatite inhibiting cancer cells. Using the hybridization in situ method to detect the expression of the telomerase gene of human hepatocellular carcinoma cells treated with the nano-apatite for 4 h at 37 ° C. The hybridization in situ showed that the cytoplasm of the positive cells was stained in nigger-brown. The positive cell rate of the control group was 88.49%, the cisplatin group was 25.6%, the nano-apatite group was 63.6%. The activity of telomerase gene both both declined declined comparing with the control group and the difference had significance (p <0.05, p <0.01). The nano-apatite obviously inhabit the expression of the telomerase gene of human hepatocellular carcinoma cells.