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目的:通过对乳铁蛋白生物学特性的研究,分析其在人脑胶质瘤中的表达及意义。方法:通过免疫组化方法测定10例非肿瘤脑组织和46例胶质瘤(其中低级别胶质瘤27例(Ⅰ级和Ⅱ级)和高级别胶质瘤19例(Ⅲ级和Ⅳ级)中乳铁蛋白的表达情况并分析二者的相关性。用RT-PCR技术及Western-blot方法测定非肿瘤脑组织、低级别组胶质瘤和高级别胶质瘤组乳铁蛋白的表达量并分析二者的相关性。结果:乳铁蛋白在非肿瘤脑组织及不同级别胶质瘤中的表达情况不同,其在低级别胶质瘤中有表达,略低于非肿瘤脑组织;而在高级别胶质瘤中表达显著减少或者表达不明显,明显低于低级别胶质瘤和非肿瘤脑组织(免疫组化、RT-PCR及Western-Blot的P值分别为P=0.001、P=0.003、P=0.004)。结论:乳铁蛋白在非肿瘤脑组织和不同级别胶质瘤中的表达有差异,提示乳铁蛋白参与了人脑胶质瘤发生与发展的过程。高级别胶质瘤的高度恶性和高侵袭性可能与乳铁蛋白表达的下调从而丧失对肿瘤细胞的抑制作用相关。
Objective: To study the biological characteristics of lactoferrin and analyze its expression in human glioma and its significance. Methods: Ten cases of non-tumor brain tissues and 46 cases of gliomas (including 27 cases of low grade gliomas (grade Ⅰ and Ⅱ) and 19 cases of high grade gliomas (Ⅲ and Ⅳ) were detected by immunohistochemistry ) In lactoferrin and its correlation.The expression of lactoferrin in non-tumor brain tissue, low-grade glioma and high-grade glioma was determined by RT-PCR and Western-blot And analyze the correlation between the two.Results: The expression of lactoferrin in non-tumor brain tissue and different grades of glioma is different, it is expressed in low-grade glioma, slightly lower than non-tumor brain tissue; However, the expression in high grade glioma was significantly decreased or the expression was not obvious, which was significantly lower than that in low grade glioma and non tumor neoplasm (P = 0.001 for RT-PCR and Western-Blot, P = 0.003, P = 0.004) .Conclusion: The expression of lactoferrin in non-tumor brain tissue and different grades of glioma are different, suggesting that lactoferrin is involved in the process of human glioma development and progression. The high degree of malignancy and high invasiveness of gliomas may be associated with the downregulation of lactoferrin expression Inhibition of tumor cell associated.