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研究乌贼墨对小ＩＬ—２（ｉｎｔｅｒｌｅｕｋｉｎ—２）的诱生作用。用乌贼墨连续５ｄ给予ＢＡＬＢ／ｃ，小鼠灌胃后，取其抗凝血，分得淋巴细胞，制备ＩＬ— ２待检上清，通过 ＭＴＴ（［３─（４，５— Ｄｉｍｅｔｈｙｌｔｈｉａｚｏｌ— ２ｙＬ）─２，５ｄｉｐｈｅｎｙｌｔ ｅｔｒａｚｏｌｉｕｍ ｂｒｏｍｉｄｅ］）比色法测其ＩＬ—２分泌活性。结果表明：致敏后２４ｈ小鼠血液中 ＩＬ—２活性升高达５０％以上，与对照组比较有明显差异（Ｐ＜０．０１），持续至４８ｈ开始呈下降趋势，９６ｈ后恢复正常。证实口服乌贼墨为诱生小鼠ＩＬ─２的唯一途径。
To study the effect of sepia on the induction of IL-2 (interleukin-2). After being given BALB/c for 5 days using sepia ink, the mice were given intragastrically and anticoagulated blood was collected to obtain lymphocytes. The supernatant of IL-2 was prepared and passed through MTT ([3—(4,5—Dimethylthiazol—2yL) )-2,5diphenylt etrazolium bromide]) colorimetric assay for IL-2 secretion activity. The results showed that the IL-2 activity in the blood of mice was increased by more than 50% at 24 h after sensitization, which was significantly different from that in the control group (P<0.01). It continued to decrease until 48 h and returned to normal after 96 h. It was confirmed that oral squid ink is the only way to induce IL-2 in mice.