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目的:研究苄普地尔对大鼠海马CA1区锥体细胞钠电流的影响。方法:膜片箝全细胞记录技术。结果:苄普地尔显著降低大鼠海马CA1区锥体细胞钠电流,作用呈时间及浓度依赖关系。苄普地尔10μmol·L~(-1)的半阻断时间约为10min。IC_(50)为2.6(2.3-2.9)μmol·L~(-1)。苄普地尔10μmol·L~(-1)右移最大电流的激活电位10mV,左移半失活膜电位18mV,表明其电压依赖地影响钠通道的激活和失活过程。结论:苄普地尔阻断大鼠海马CA1区锥体细胞钠电流,可能是其抗脑缺血机制之一。
Objective: To study the effect of beupipal on sodium currents in pyramidal cells of hippocampal CA1 area of rats. Methods: patch clamp whole cell recording technology. RESULTS: Bepridil significantly decreased the sodium currents in pyramidal cells in hippocampal CA1 region of rats in a time and concentration dependent manner. The half-block time of bepridil 10μmol·L -1 was about 10min. IC_ (50) was 2.6 (2.3-2.9) μmol·L -1. The potentials of 10μol · L ~ (-1) maximal shift of bepupil and 10mV of left shifted half-inducible membrane potentials (LVs), indicating that they can affect the activation and inactivation of sodium channels in a voltage-dependent manner. Conclusion: Beppudeside may block the sodium currents of pyramidal cells in hippocampal CA1 region of rats, which may be one of its anti-ischemic mechanisms.