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Lindner等早就提出了甾体分子的功能团和牛血清白蛋白(BSA)连接有获得高度专一性的免疫血清的可能性。后来的实践完全证明了这点。用放射免疫测量血相中的甾体化合物的含量就不必像以前那样要对血相提取物进行层析分离而可以直接测定。因此近十年以来,甾体化合物的放射免疫测定(radioimmunoassay,RIA)得到了迅速的发展。一般来说,甾体分子结构不同,与血清蛋白结合后对其有关活性具有最大的影响,所有动物注射了17β-雌二醇的免疫血清后产生了合适的高浓度和高度专一性的免疫血清。17β-雌二醇的免疫血清是从注射了17β-雌二醇-6-(O-羧甲基)肟牛血清白蛋白(Ⅰ)*的兔中获得的。我们制备了化合物(Ⅰ),以17β-雌二醇(Ⅱ)为原料,用醋酐吡啶乙酰化得17β-雌
Lindner et al. Have long proposed the possibility that functional groups of steroid molecules may be linked to bovine serum albumin (BSA) to obtain highly specific immune sera. Later practice fully proved this point. It is not necessary to measure the content of the steroid in the blood by radioimmunoassay, as it is not necessary to perform chromatographic separation of the blood-phase extract as before. Therefore, the radioimmunoassay (RIA) of steroids has been rapidly developed in recent ten years. In general, the steroid molecules have different structures and have the greatest impact on their activity when combined with serum proteins. All animals injected with 17β-estradiol in serum gave a suitably high concentration and high specificity of immunity serum. 17β-estradiol was obtained from rabbits injected with 17β-estradiol-6- (O-carboxymethyl) oxime bovine serum albumin (I) *. We prepared the compound (Ⅰ), 17β-estradiol (Ⅱ) as raw materials, acetic anhydride pyridine acetylation 17β-feminine