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The objectives of this study were to investigate antimicrobial resistance of Streptococcus uberis and Streptococcus parauberis isolated from cows with bovine clinical mastitis in China and to examine the distribution of resistance- and virulence-related gene patts. Antimicrobial susceptibility was determined by the E-test. Genes encoding antimicrobial resistance and invasiveness factors were examined by PCR. A total of 27 strains were obtained from 326 mastitis milk samples. Streptococcus?parauberis isolates (n=11) showed high resistance to erythromycin (90.9％), followed by tetracycline (45.5％), chloramphenicol (36.4％) and clindamycin (27.3％). Streptococcus?uberis isolates (n=16) were highly resistant to tetracycline (81.3％) and clindamycin (62.5％). Both species were susceptible to ampicillin. The most prevalent resistance gene in S.?uberis was tetM (80.0％), followed by blaZ (62.5％) and ermB (62.5％). However, tetM, blaZ, and ermB genes were only found in 27.3, 45.5, and 27.3％, respectively, of S.?parauberis. In addition, all of the isolates carried at least one selected virulence-related gene. The most prevalent virulence-associated gene patt in the current study was sua+pauA/skc+gapC+hasC detected in 22.2％ of the strains. One S.?uberis strain carried 7 virulence-associated genes and belonged to the sua+pauA/skc+gapC+cfu+hasA+hasB+hasC patt. More than 59.3％ of analysed strains carried 4 to 7 virulence-related genes. Our findings demonstrated that S.?parauberis and S.?uberis isolated from clinical bovine mastitis cases in China exhibited diverse molecular ecology, and that the strains were highly resistant to antibiotics commonly used in the dairy cow industry. The data obtained in the current study contribute to a better understanding of the pathogenesis of bacteria in mastitis caused by these pathogens, and the findings are relevant to the development of multivalent vaccines and targeted prevention procedures.