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目的:以骨髓瘤细胞株RPMI8226为实验对象,观察丙戊酸钠(valproic acid,VPA)和硼替佐米(bortezomib,BZ)对此细胞株的增殖及凋亡的诱导情况。方法:实验分组如下:对照组,VPA单药组(1.0 mmol/L),BZ单药A组(10.0 nmol/L),BZ单药B组(20.0 nmol/L),BZ单药C组(35.0 nmol/L),联合用药A组(VPA1.0 mmol/L+BZ10.0 nmol/L),联合用药B组(VPA 1.0 mmol/L+BZ20.0 nmol/L),联合用药C组(VPA 1.0 mmol/L+BZ 35.0 nmol/L)。用MTT技术检测细胞增殖抑制情况;流式细胞仪检测凋亡比例。结果:丙戊酸钠与硼替佐米单用对RPMI8226细胞株细胞增殖有抑制作用,有细胞凋亡,但丙戊酸钠与硼替佐米协同用药A组、B组、C组增殖抑制可达75.1%及凋亡情况可达68.9%(P<0.01)。结论:丙戊酸钠与硼替佐米协同用药后对RPMI8226细胞增殖抑制及诱导凋亡作用更显著,丙戊酸钠对硼替佐米有增敏作用。
OBJECTIVE: To investigate the effects of valproic acid (VPA) and bortezomib (BZ) on the proliferation and apoptosis of myeloma cell line RPMI8226. Methods: The experimental groups were as follows: control group, VPA single group (1.0 mmol / L), BZ single group 10.0 mmol / L, BZ single group B 20.0 nmol / L, BZ single group C 35.0 nmol / L), combination group A (VPA1.0 mmol / L and BZ10.0 nmol / L), combination group B (VPA 1.0 mmol / L and BZ20.0 nmol / L) VPA 1.0 mmol / L + BZ 35.0 nmol / L). MTT technique was used to detect the inhibition of cell proliferation; flow cytometry was used to detect the percentage of apoptosis. Results: Sodium valproate and bortezomib alone could inhibit the proliferation of RPMI8226 cells and induce cell apoptosis. However, the synergistic effects of sodium valproate and bortezomib on the proliferation of AML, B and C groups were inhibited 75.1% and apoptosis reached 68.9% (P <0.01). CONCLUSION: Sodium valproate synergizes with bortezomib in inhibiting the proliferation and inducing apoptosis of RPMI8226 cells. Sodium valproate sensitizes bortezomib.