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本文报道了对我国著名的南药之一的巴戟天(Morinda officinalis How)试管苗培养的研究结果,为这种名贵药材的优良种苗的快速大量繁殖和抗性良种的选育提供了新途径。结果表明:(1)对巴戟天外植体(顶芽、腋芽、茎段和叶片)的较好的消毒方法是:外植体经自来水洗净→70%乙醇预处理5秒钟→0.1%HgCl_2溶液中消毒10至15分钟→无茵水漂洗5次,其消毒效果为70～75%,对外植体基本无损伤; (2)顶芽和腋芽的培养效果比茎段和叶片的培养效果好,因此应优先择选顶芽和腋芽为巴戟天试管苗的起始培养物; (3)试管苗的最佳培养程序为:经消毒的顶芽或腋芽在增殖培养基(MS+NAA0.5+BA6.0+GA。1.0+3%蔗糖,单位:mg/L.下同。)中培养40天后可分化出丛生的不定芽(分化率为86.7%),将这些不定芽转入生根培养基(1/2MS+IAA0.2+NAA0.4+1%蔗糖)中培养30天即可分化生根形成完整的巴戟天试管苗,其根分化率为84.6%。试管苗经过两周的沙培室内炼苗后即可移栽盆植,约1～2个月后即可移栽至露天土地种植,移栽成活率大干90%。
This paper reports the results of the research on the culture of Morinda officinalis How, one of the famous southern medicines in China, and provides a new breed for the rapid propagation of elite seedlings and the breeding of resistant varieties. way. The results showed that: (1) The best method of disinfection of Morinda officinalis explants (apical buds, axillary buds, stem segments and leaves) was as follows: Explants were washed with tap water → 70% ethanol pretreatment for 5 seconds → 0.1% HgCl 2 solution was disinfected for 10 to 15 minutes and rinsed with sterile water for 5 times. The disinfection effect was 70 to 75% and the explants were basically free of damage. (2) The culture effect of the apical buds and axillary buds was better than that of stem segments and leaves. Good, so the first bud and axillary bud should be the first choice for the initial culture of Morinda officinalis plantlets; (3) The best culture procedure for the test-tube plantlets is: Sterilized apical buds or axillary buds in proliferation medium (MS+NAA0 .5+BA6.0+GA.1.0+3% sucrose, unit: mg/L. Same as above.) After 40 days of cultivation, adventitious shoots (86.7%) were differentiated, and these adventitious buds were transferred to The rooting medium (1/2MS+IAA0.2+NAA0.4+1% sucrose) can be cultured for 30 days to induce root formation to form a complete Morinda testis seedling, with a root differentiation rate of 84.6%. After two weeks of inoculation, the test-tube seedlings can be transplanted and planted. After about 1 to 2 months, they can be transplanted to open-air land for planting, and the survival rate for transplanting is 90%.