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将携带ＴＮＦ基因的逆转录病毒上清与小鼠ＭΦ共同孵育，使ＴＮＦ基因导入ＭΦ中，结果发现，ＴＮＦ基因转染的巨噬细胞（ＭΦ─ＴＮＦ＋）分泌ＴＮＦ的水平明显高于正常ＭΦ和转染对照基因的ＭΦ。研究中还发现，ＭΦ─ＴＮＦ＋对Ｐ８１５肿瘤细胞的杀伤率和吞噬力明显高于对照组，用抗ＴＮＦ单抗可明显抑制ＭΦ─ＴＮＦ＋的体外杀伤活性。由此表明，ＭΦ杀伤活性的升高系细胞经基因导入后表达的ＴＮＦ介导的。
The TNF gene-carrying retrovirus supernatant was co-incubated with mouse MΦ to induce TNF gene into MΦ. The results showed that TNF gene transfected macrophages (MΦ-TNF+) secreted TNF at a significantly higher level than normal MΦ and The MΦ of the control gene was transfected. It was also found in the study that the killing rate and phagocytic activity of MΦ-TNF+ against P815 tumor cells were significantly higher than that of the control group. The anti-TNF monoclonal antibody can significantly inhibit the in vitro killing activity of MΦ-TNF+. This indicates that the increased killing activity of MΦ is mediated by TNF expressed after the gene has been introduced into cells.